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Proceedings 2017, 1(10), 991; doi:10.3390/proceedings1100991

Investigation of Cytotoxic Effect of Origanum Minutiflorum on Cancer Cells

1
Department of Medicinal Pharmacology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
2
Department of Histology and Embryology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
3
Department of Medicinal Biochemistry, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
4
Department of Medicinal Biology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
5
Department of Medicinal Microbiology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
6
Department of Biology, Faculty of Arts and Sciences, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
7
Department of Histology and Embryology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 45140, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November, 2017.
*
Author to whom correspondence should be addressed.
Published: 15 November 2017
Download PDF [167 KB, uploaded 15 November 2017]

Abstract

The number of breast cancer cases have increased gradually in women. During treatment of the breast cancer, chemotherapy needs adjuvant agents. Medicinal plants have an anticancerogenic effect in such cases. Our aim is to search the cytotoxic effect of Origanum minutiflorum O. Schwarz & P.H. Davis on breast cancer cells in vitro conditions. For this purpose MDA-MB-231 and MCF-7 breast cancer cell lines and adipose tissue derived mesenchymal stem cells were used. Oregano oil from Origanum minutiflorum was exposed to the all cells and IC50 dose was calculated by MTT assay. The immunocytochemical staining of eNOS, p53, PCNA and TUNEL at IC50 dose for each cells. The results were evaluated using one-way-ANOVA by Graphpad software. The proliferation of breast cancer cells were inhibited by oregano oil. Oregano oil did not show any cytotoxic effect in mesenchymal stem cells. It was determined that cytotoxic effect of oregano oil on cancer cells was occurred through increase of eNOS and p53 staining for survival and the TUNEL labeling for apoptosis, and decreased of PCNA staining for proliferation. According to our data, oregano oil has cytotoxic effect on breast cancer cell lines, and it needs to be analysis to detect active components for other biological activities.
Keywords: breast cancer; MCF-7; MDA-MB-231; Origanum minutiflorum; cytotoxicity; oxidative stress; apoptosis breast cancer; MCF-7; MDA-MB-231; Origanum minutiflorum; cytotoxicity; oxidative stress; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Özkan, O.; Aydemir, I.; Sarı, İ.; Dönmez, G.; Kırık, F.E.; Savran, A.; Tuğlu, M.İ. Investigation of Cytotoxic Effect of Origanum Minutiflorum on Cancer Cells. Proceedings 2017, 1, 991.

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