Investigation of Cytotoxic Effect of Origanum minutiflorum on Cancer Cells †
by
Oktay Özkan
1,*, 
Işıl Aydemir
2,
İsmail Sarı
3,
Gonca Dönmez
4,
Fatma Esin Kırık
5,
Ahmet Savran
6 and
Mehmet İbrahim Tuğlu
7 1
Department of Medicinal Pharmacology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
2
Department of Histology and Embryology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
3
Department of Medicinal Biochemistry, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
4
Department of Medicinal Biology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
5
Department of Medicinal Microbiology, Faculty of Medicine, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
6
Department of Biology, Faculty of Arts and Sciences, Niğde Ömer Halisdemir University, Niğde 51240, Turkey
7
Department of Histology and Embryology, Faculty of Medicine, Manisa Celal Bayar University, Manisa 45140, Turkey
*
Author to whom correspondence should be addressed.
†
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
Proceedings 2017, 1(10), 991; https://doi.org/10.3390/proceedings1100991
Published: 15 November 2017
(This article belongs to the Proceedings of Proceedings of the 2nd International Conference on Natural Products for Cancer Prevention and Therapy)
Abstract
:The number of breast cancer cases have increased gradually in women. During treatment of the breast cancer, chemotherapy needs adjuvant agents. Medicinal plants have an anticancerogenic effect in such cases. Our aim is to search the cytotoxic effect of Origanum minutiflorum O. Schwarz & P.H. Davis on breast cancer cells in vitro conditions. For this purpose MDA-MB-231 and MCF-7 breast cancer cell lines and adipose tissue derived mesenchymal stem cells were used. Oregano oil from Origanum minutiflorum was exposed to the all cells and IC50 dose was calculated by MTT assay. The immunocytochemical staining of eNOS, p53, PCNA and TUNEL at IC50 dose for each cells. The results were evaluated using one-way-ANOVA by Graphpad software. The proliferation of breast cancer cells were inhibited by oregano oil. Oregano oil did not show any cytotoxic effect in mesenchymal stem cells. It was determined that cytotoxic effect of oregano oil on cancer cells was occurred through increase of eNOS and p53 staining for survival and the TUNEL labeling for apoptosis, and decreased of PCNA staining for proliferation. According to our data, oregano oil has cytotoxic effect on breast cancer cell lines, and it needs to be analysis to detect active components for other biological activities.
Keywords:
breast cancer; MCF-7; MDA-MB-231; Origanum minutiflorum; cytotoxicity; oxidative stress; apoptosisConflicts of Interest
The authors declare no conflict of interest.
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MDPI and ACS Style
Özkan, O.; Aydemir, I.; Sarı, İ.; Dönmez, G.; Kırık, F.E.; Savran, A.; Tuğlu, M.İ. Investigation of Cytotoxic Effect of Origanum minutiflorum on Cancer Cells. Proceedings 2017, 1, 991. https://doi.org/10.3390/proceedings1100991
AMA Style
Özkan O, Aydemir I, Sarı İ, Dönmez G, Kırık FE, Savran A, Tuğlu Mİ. Investigation of Cytotoxic Effect of Origanum minutiflorum on Cancer Cells. Proceedings. 2017; 1(10):991. https://doi.org/10.3390/proceedings1100991
Chicago/Turabian StyleÖzkan, Oktay, Işıl Aydemir, İsmail Sarı, Gonca Dönmez, Fatma Esin Kırık, Ahmet Savran, and Mehmet İbrahim Tuğlu. 2017. "Investigation of Cytotoxic Effect of Origanum minutiflorum on Cancer Cells" Proceedings 1, no. 10: 991. https://doi.org/10.3390/proceedings1100991
