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Proceedings 2017, 1(10), 1076; doi:10.3390/proceedings1101076

Is Acteosid Effects On Colon Cancer Stem Cells via Inflamation and/or Apoptosis?

1
Department of Histology and Embryology, Faculty of Medicine, Manisa Celal Bayar University, 45140 Manisa, Turkey
2
Department of Biology, Faculty of Art and Life Sciences, Manisa Celal Bayar University, 45140 Manisa, Turkey
3
Experimental Health Research Center of Health Sciences, Near East University, 99138 Mersin, Turkey
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November 2017.
*
Author to whom correspondence should be addressed.
Published: 13 November 2017
Download PDF [140 KB, uploaded 13 November 2017]

Abstract

Colorectal cancer, as one of the main health problems worldwide. Cancer stem cells (CSCs) are called tumor-initiating cells involved in tumour heterogeneity and dormancy. CSCs may cause drug resistance, metastasis and relapse of primary and metastatic cancers. Dysregulation of survive and their cross-talk with other cell pathways may alternative way for effective treatment. We aimed whether acteosid, affects stemness and inflamatuvary prosess in primary (HCT-116) and metastatic (Colo-741) colon CSCs. CSCs were obtained from both type of colon cancer cell lines using MINIMACS system using anti-CD133. CD133+ and CD133- cells from Colo-741 and HCT-116 were cultured without or with Acteosid for 48 h. Distribution of Caspase-3, Bcl, Bax, IL-1β, TNF-α, IL-6, IL8 and IL-10 analyzed using indirect immunohistoperoxidase technique. Acteosid increased the intensity of Bax/Bcl ratio on CD133+, CD133- Colo-741 cells, there wasn’t differences of Caspase-3 immunoreactivity. Oct-4 was also decreased in CD133+ and CD133- Colo-741 cells. Caspase 3 was slightly increased CD133+ cells after incubation with Acteoside. In conclusion, it has been observed that the viability and stemness of the HCT-116 was increased after Acteoside, these properties were not changed in CD133+ Colo-741 cells. Therefore inhibition of ROS may play a role during CSCs stemness and viability.
Keywords: colon cancer; cancer stemness; acteosid; inflammation; apoptosis colon cancer; cancer stemness; acteosid; inflammation; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Firat, F.; Türkoğlu, C.; Kurt, F.Ö.; Vatansever, H.S. Is Acteosid Effects On Colon Cancer Stem Cells via Inflamation and/or Apoptosis? Proceedings 2017, 1, 1076.

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