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Inventions 2018, 3(2), 34; https://doi.org/10.3390/inventions3020034

Rapid Paper-Based System for Human Serum Creatinine Detection

1
Graduate Institute of Materials Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
2
Department of Biomechatronics Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
3
Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan
4
Department of Internal Medicine, College of Medicine, National Cheng Kung University and Hospital, Tainan 704, Taiwan
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 9 April 2018 / Revised: 21 May 2018 / Accepted: 21 May 2018 / Published: 28 May 2018
(This article belongs to the Special Issue Microfluidics and Nanofluidics)
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Abstract

An integrated system consisting of a paper-based chip and a smart detection device is proposed for determining the human serum creatinine concentration based on Jaffé reaction theory. In the proposed approach, the reaction zone of the paper-based chip is implanted with picric acid and NaOH reagent and dried at 35 °C for 20 min. Human serum creatinine is dripped onto the reaction zone of the chip. A Jaffé reaction is induced by heating the chip at 37 °C for 5 min and the creatinine concentration is then derived by analyzing the RGB (red, green and blue) intensity of the resulting Janovsky complex using self-written analysis software installed on a smartphone. The validity of the proposed method is demonstrated using control samples with creatinine concentrations ranging from 0.2~8 mg/dL. The detection results obtained for 32 real-world creatinine samples are shown to be in excellent agreement with those obtained using a standard macroscale method (R2 = 0.9994). Overall, the results show that the proposed system provides a compact, low-cost and reliable approach for human serum creatinine concentration detection. View Full-Text
Keywords: paper-based chip; smart detection device; creatinine; Jaffé reaction paper-based chip; smart detection device; creatinine; Jaffé reaction
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Fu, L.-M.; Tseng, C.-C.; Ju, W.-J.; Yang, R.-J. Rapid Paper-Based System for Human Serum Creatinine Detection. Inventions 2018, 3, 34.

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