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Methods Protoc. 2018, 1(3), 27; https://doi.org/10.3390/mps1030027

A Rapid Bacteriophage DNA Extraction Method

Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg C, Denmark
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Received: 1 June 2018 / Revised: 6 July 2018 / Accepted: 24 July 2018 / Published: 27 July 2018
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Abstract

Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform. View Full-Text
Keywords: phage; fast; DNA extraction; spin column; genome; sequencing phage; fast; DNA extraction; spin column; genome; sequencing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Jakočiūnė, D.; Moodley, A. A Rapid Bacteriophage DNA Extraction Method. Methods Protoc. 2018, 1, 27.

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