Porous Polylactide Microparticles as Effective Fillers for Hydrogels
Abstract
:1. Introduction
- The development of composite hydrogels based on a natural polymer filled by a biocompatible filler containing active biomolecules. For example, a novel injectable nanocomposite was designed as a sustained-release platform for cartilage regeneration through the integration of a chitosan-based hydrogel, articular cartilage stem cells (ACSCs) and mesoporous SiO2 nanoparticles loaded with anhydroicaritin (AHI) [9].
1.1. Collagen and Collagen Gels
1.2. Chitosan and Chitosan Gels
1.3. Composite Gels with Porous Microparticles
1.4. C-Phycocyanin
2. Materials and Methods
2.1. Materials
2.1.1. Porous Polylactide Microparticles
2.1.2. Highly Concentrated Collagen Gel
2.1.3. Chitosan–Genipin Hydrogels
2.1.4. Composite Hydrogels
2.1.5. C-Phycocyanin
2.2. Experimental
2.2.1. Scanning Electron Microscope
2.2.2. Optical Microscopy
2.2.3. Ultrasound Investigation
2.2.4. Mechanical Properties
2.2.5. Fourier Transform Infrared Spectroscopy (FTIR)
2.3. Statistical Analysis
3. Results and Discussion
3.1. Structure Polylactide Particles
3.2. C-PC-Loaded Polylactide Particles
3.3. C-PC Release from the PLA Particles
3.4. Structure of Composite Hydrogels
3.4.1. Collagen–PLA Microparticle Composite Hydrogels
3.4.2. Chitosan–PLA Microparticle Composite Hydrogels
3.5. Mechanical Properties of Collagen and Chitosan Hydrogels Filled with Porous Lactide Nanoparticles
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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% of Particles in Composite | % of Image Filled with Particles |
---|---|
0 | 0 |
1 | 1 ± 0.3 |
7 | 22 ± 0.2 |
12 | 36 ± 1 |
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Zagoskin, Y.D.; Sergeeva, Y.E.; Fomina, Y.S.; Sukhinov, D.V.; Malakhov, S.N.; Osidak, E.O.; Khramtsova, E.A.; Gotovtsev, P.M.; Chvalun, S.N.; Grigoriev, T.E. Porous Polylactide Microparticles as Effective Fillers for Hydrogels. Biomimetics 2023, 8, 565. https://doi.org/10.3390/biomimetics8080565
Zagoskin YD, Sergeeva YE, Fomina YS, Sukhinov DV, Malakhov SN, Osidak EO, Khramtsova EA, Gotovtsev PM, Chvalun SN, Grigoriev TE. Porous Polylactide Microparticles as Effective Fillers for Hydrogels. Biomimetics. 2023; 8(8):565. https://doi.org/10.3390/biomimetics8080565
Chicago/Turabian StyleZagoskin, Yuriy D., Yana E. Sergeeva, Yuliya S. Fomina, Daniil V. Sukhinov, Sergey N. Malakhov, Egor O. Osidak, Elena A. Khramtsova, Pavel M. Gotovtsev, Sergei N. Chvalun, and Timofei E. Grigoriev. 2023. "Porous Polylactide Microparticles as Effective Fillers for Hydrogels" Biomimetics 8, no. 8: 565. https://doi.org/10.3390/biomimetics8080565