Temporal Changes in Flavonoid Components, Free Radical Scavenging Activities and Metabolism-Related Gene Expressions during Fruit Development in Chinese Dwarf Cherry (Prunus humilis)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Chemicals
2.2. Extraction and Determination of Flavonoids
2.3. Flavonoid Composition Determination
2.4. Antioxidant Determination
2.5. Gene Expression Analysis
2.6. Statistical Analysis
3. Results
3.1. Changes in Total Flavonoid Content during Fruit Development
3.2. Flavonoid Composition Variation during Fruit Development
3.3. Antioxidant Activity Variation during Fruit Development
3.4. Expression Analysis of Flavonoid Biosynthetic Genes during Fruit Development
3.5. Correlation Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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P. humilis Gene ID | P. humilis Gene Name | Forward Primer | Reverse Primer |
---|---|---|---|
CL2555.Contig1 | ChCHS | TACCAACAAGGGTGTTTCGC | GTGATCTCCGAGCACACAAC |
Unigene14928 | ChCHI | GAGGAGGAAGCCTTGGAGAA | TCCTCCTTCCCTTCAGTGTG |
Unigene3562 | ChF3H | TACAGGGAGAAGCTGTGCAA | TCACCTCTCTCCATCCCTCA |
Unigene6640 | ChDFR | TGTCGAAGAGCACCAGAAGT | GGCCAATCACAAGAGTTGGG |
Unigene14860 | ChLDOX | GGAAGGCTGGAGAAGGAAGT | TGAGCTTCAACACCAAGTGC |
Unigene6233 | ChUFGT | TGTTTGATGTGGCTGATGGC | CGTCGGTAATCAAGCAGGTG |
Unigene5990 | ChLAR | TGGCATCTCTGTGGGAGAAG | TTTCCGGTATGCGGTTCTCT |
Unigene7152 | ChANR | GAGGACCCTGAGAACGACAT | TCGTTCTCGTCTGTGACCAA |
Unigene17057 | ChFLS | GAGTTGAGGTCGTCATTGCC | TCAAGGACCCTCCCATGAAC |
- | ChActin | GCAGCGACTGAAGACATACA | GTGGCATTAGCAAGTTCCTC |
Developmental Stages | Nongda 3 (mg/g RE·FW) | Jinou 1 (mg/g RE·FW) | Nongda 5 (mg/g RE·FW) |
---|---|---|---|
YFS | 22.33 ± 0.88 a | 47.21 ± 1.01 a | 12.33 ± 0.92 a |
PHS | 16.98 ± 0.99 b | 24.33 ± 1.56 b | 9.97 ± 1.05 b |
FES | 12.56 ± 0.67 c | 20.65 ± 0.93 c | 9.56 ± 0.98 b |
CCS | 11.43 ± 0.33 cd | 13.23 ± 1.23 d | 9.21 ± 0.65 b |
FRS | 10.00 ± 0.42 e | 11.94 ± 0.26 de | 7.23 ± 0.32 c |
Cultivar | Developmental Stages | DPPH (mg TE/g) | FRAP (mg TE/g) | ABTS (mg TE/g) |
---|---|---|---|---|
Nongda 3 | YFS | 28.25 ± 0.39 a | 53.54 ± 0.77 a | 78.03 ± 1.01 a |
PHS | 23.22 ± 0.41 b | 45.02 ± 0.59 b | 68.41 ± 0.74 b | |
FES | 6.13 ± 0.20 c | 14.73 ± 0.34 c | 31.23 ± 0.57 c | |
CCS | 4.10 ± 0.16 d | 10.88 ± 0.44 d | 9.82 ± 0.46 d | |
FRS | 3.33 ± 0.18 e | 9.07 ± 0.50 e | 8.18 ± 0.37 e | |
Jinou 1 | YFS | 36.21 ± 0.53 a | 88.14 ± 1.28 a | 121.02 ± 2.04 a |
PHS | 35.88 ± 0.62 a | 87.92 ± 1.52 a | 120.41 ± 1.98 a | |
FES | 7.23 ± 0.14 b | 18.31 ± 0.55 b | 43.23 ± 0.63 b | |
CCS | 5.12 ± 0.11 c | 16.80 ± 0.47 c | 14.82 ± 0.58 c | |
FRS | 4.04 ± 0.22 d | 11.23 ± 0.53 d | 12.18 ± 0.49 d | |
Nongda 5 | YFS | 23.72 ± 0.60 a | 27.21 ± 0.57 a | 60.01 ± 1.04 a |
PHS | 19.65 ± 0.59 b | 25.76 ± 0.55 b | 57.65 ± 0.89 b | |
FES | 5.72 ± 0.23 c | 12.23 ± 0.45 c | 23.31 ± 0.58 c | |
CCS | 3.53 ± 0.14 d | 7.92 ± 0.43 d | 7.44 ± 0.45 d | |
FRS | 2.84 ± 0.12 e | 6.88 ± 0.46 e | 6.57 ± 0.46 e |
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Han, H.; Zhang, L.; Liu, S.; Li, N.; Huo, J.; Mu, X. Temporal Changes in Flavonoid Components, Free Radical Scavenging Activities and Metabolism-Related Gene Expressions during Fruit Development in Chinese Dwarf Cherry (Prunus humilis). Horticulturae 2023, 9, 1040. https://doi.org/10.3390/horticulturae9091040
Han H, Zhang L, Liu S, Li N, Huo J, Mu X. Temporal Changes in Flavonoid Components, Free Radical Scavenging Activities and Metabolism-Related Gene Expressions during Fruit Development in Chinese Dwarf Cherry (Prunus humilis). Horticulturae. 2023; 9(9):1040. https://doi.org/10.3390/horticulturae9091040
Chicago/Turabian StyleHan, Hongyan, Lingjuan Zhang, Shan Liu, Na Li, Jianxin Huo, and Xiaopeng Mu. 2023. "Temporal Changes in Flavonoid Components, Free Radical Scavenging Activities and Metabolism-Related Gene Expressions during Fruit Development in Chinese Dwarf Cherry (Prunus humilis)" Horticulturae 9, no. 9: 1040. https://doi.org/10.3390/horticulturae9091040