Time-Dependent Production of the Bioactive Peptides Endolides A and B and the Polyketide Mariline A from the Sponge-Derived Fungus Stachylidium bicolor 293K04
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Material
2.2. General Experimental Procedures
2.2.1. Cultivation Procedures
2.2.2. Extraction of Cultures
2.3. Ultraviolet-Liquid Chromatography-Mass Spectrometry (UV-LC-MS) Analysis
2.3.1. Low Resolution UV-LC-MS
2.3.2. High Resolution UV-LC-MS
2.4. Methods for Time Course Measurements
2.5. Radial Growth and Optimum Temperature Growth Measurements in Solid Medium
2.6. Calibration Curves
3. Results
3.1. Production of Endolides by S. bicolor 293K04 during Short Incubation Times
3.2. Time-Course Study for S. bicolor 293K04
3.2.1. Optimal Temperature for Growth of S. bicolor
3.2.2. pH and Biomass Variation during the Time Course
3.2.3. Endolides Production
3.2.4. Mariline A1/A2 Production
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Almeida, C.; Bills, G.; González-Menéndez, V.; Martin, J.; Tormo, J.R.; Genilloud, O. Time-Dependent Production of the Bioactive Peptides Endolides A and B and the Polyketide Mariline A from the Sponge-Derived Fungus Stachylidium bicolor 293K04. Fermentation 2017, 3, 45. https://doi.org/10.3390/fermentation3030045
Almeida C, Bills G, González-Menéndez V, Martin J, Tormo JR, Genilloud O. Time-Dependent Production of the Bioactive Peptides Endolides A and B and the Polyketide Mariline A from the Sponge-Derived Fungus Stachylidium bicolor 293K04. Fermentation. 2017; 3(3):45. https://doi.org/10.3390/fermentation3030045
Chicago/Turabian StyleAlmeida, Celso, Gerald Bills, Víctor González-Menéndez, Jesús Martin, José R. Tormo, and Olga Genilloud. 2017. "Time-Dependent Production of the Bioactive Peptides Endolides A and B and the Polyketide Mariline A from the Sponge-Derived Fungus Stachylidium bicolor 293K04" Fermentation 3, no. 3: 45. https://doi.org/10.3390/fermentation3030045