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Fermentation 2015, 1(1), 13-23; doi:10.3390/fermentation1010013

Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions

1
Department of Food Science, Harper Adams University, Newport, Shropshire TF10 8NB, UK
2
Alltech Biotechnology, Nicholasville, KY 40536, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Hiroshi Kitagaki
Received: 22 May 2015 / Revised: 20 July 2015 / Accepted: 24 July 2015 / Published: 30 July 2015
(This article belongs to the Special Issue Beneficial Fermentation Microbes and Their Functional Compounds)
View Full-Text   |   Download PDF [397 KB, uploaded 30 July 2015]   |  

Abstract

Cultivation of Escherichia coli on wheat-bran substrate under various Solid-State Fermentation (SSF) conditions was evaluated for phytase yield along with the enzyme activity profile as a potential, low-cost alternative to submerged-liquid fermentation. The maximum phytase activity achieved by E. coli was 350 ± 50 SPU of phytase activity per gram of bran, incubated for 96 h with a substrate bed moisture content of 70% (w/v) at 37 °C with a relative air humidity of 90%, and supplemented with 10% (w/w bran) Luria-Bertani broth powder which translates into a 300% increase in phytase activity compared with an un-supplemented culture. The greatest improvements in phytase yield were associated with nutrient supplementation and the optimization of initial substrate moisture content. E. coli production of phytase utilizing solid-state fermentation technology was shown to be feasible utilizing the low-cost agro-residue wheat bran as substrate. Furthermore, the effect of pH and temperature on phytase activity was monitored from pH 2.5 to pH 7.5, and for temperatures ranging from 20 °C to 70 °C. Optimal phytase activity was at pH 5.5 and 50 °C when produced under the SSF optimized conditions. View Full-Text
Keywords: E. coli; enzyme; optimization; phytase; solid state fermentation E. coli; enzyme; optimization; phytase; solid state fermentation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

McKinney, K.; Combs, J.; Becker, P.; Humphries, A.; Filer, K.; Vriesekoop, F. Optimization of Phytase Production from Escherichia coli by Altering Solid-State Fermentation Conditions. Fermentation 2015, 1, 13-23.

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