The Characterization and the Biological Activity of Phytotoxin Produced by Paraphoma radicina
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fungal Cultures and Cell-Free Culture Filtrate (CFCF) Production
2.2. Extraction of Crude Toxin from the Pathogen Culture
2.3. Effect of Heat Treatment on Stability of Crude Toxin Activity
2.4. Detached Leaf Bioassay
2.5. Effect of the Crude Toxin on Susceptible and Resistant Cultivars
2.5.1. Effect of Crude Toxin in Water Medium
2.5.2. Effect of Crude Toxin in MS Medium
2.6. Transmission Electron Microscopy
2.7. Host Selectivity Test
2.8. Gas Chromatography-Mass Spectrometry
2.9. Data Analysis
3. Results
3.1. Toxin Production
3.2. Crude Toxin Extraction
3.3. Effect of the Crude Toxin on Susceptible and Resistant Cultivars
3.3.1. Effect of Crude Toxin in Water Medium
3.3.2. Effect of Crude Toxin in MS Medium
3.4. Transmission Electron Microscopy
3.5. Host Selectivity Test
3.6. Gas Chromatography-Mass Spectrometry
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Cao, S.; Liang, Q.W. Paraphoma root rot of alfalfa (Medicago sativa) in Inner Mongolia, China. Plant Pathol. 2020, 69, 231–239. [Google Scholar] [CrossRef]
- Aveskamp, M.M.; de Gruyter, J. Highlights of the Didymellaceae: A polyphasic approach to characterise Phoma and related pleosporalean genera. Stud. Mycol. 2010, 65, 1–60. [Google Scholar] [CrossRef] [PubMed]
- De Gruyter, J.; Woudenberg, J.H.C. Systematic reappraisal of species in Phoma section Paraphoma, Pyrenochaeta and Pleurophoma. Mycologia 2010, 102, 1066–1081. [Google Scholar] [CrossRef] [PubMed]
- Dang, S.Z.; Cao, S. Specific primers of Paraphoma radicina which causes alfalfa Paraphoma root rot. Eur. J. Plant Pathol. 2022, 162, 489–499. [Google Scholar] [CrossRef]
- Xu, D.; Xue, M. Phytotoxic Secondary Metabolites from Fungi. Toxins 2021, 13, 261. [Google Scholar] [CrossRef]
- Duke, S.O.; Dayan, F.E. Modes of Action of Microbially-Produced Phytotoxins. Toxins 2011, 3, 1038–1064. [Google Scholar] [CrossRef]
- Wolpert, T.J.; Dunkle, L.D. Host-selective toxins and avirulence determinants: What’s in a name? Annu. Rev. Phytopathol. 2002, 40, 251–285. [Google Scholar] [CrossRef]
- Chen, H.; Singh, H. An exploration on the toxicity mechanisms of phytotoxins and their potential utilities. Crit. Rev. Environ. Sci. Technol. 2022, 52, 395–435. [Google Scholar] [CrossRef]
- Möbius, N.; Hertweck, C. Fungal phytotoxins as mediators of virulence. Curr. Opin. Plant Biol. 2009, 12, 390–398. [Google Scholar] [CrossRef]
- Oliver, R.P.; Solomon, P.S. Recent fungal diseases of crop plants: Is lateral gene transfer a common theme? Mol. Plant-Microbe Interact. 2008, 21, 287–293. [Google Scholar] [CrossRef]
- Daub, M.E.; Chung, K.R. Photoactivated Perylenequinone Toxins in Plant Pathogenesis. In Plant Relationships; Deising, H.B., Ed.; Springer: Berlin/Heidelberg, Germany, 2009; pp. 201–219. [Google Scholar]
- Liu, S.; Li, J. Fusaric acid instigates the invasion of banana by Fusarium oxysporum f. sp. cubense TR4. New Phytol. 2020, 225, 913–929. [Google Scholar] [CrossRef] [PubMed]
- Dong, X.; Ling, N. Fusaric acid is a crucial factor in the disturbance of leaf water imbalance in Fusarium-infected banana plants. Plant Physiol. Biochem. 2012, 60, 171–179. [Google Scholar] [CrossRef] [PubMed]
- Berestetskiy, A.O. A review of fungal phytotoxins: From basic studies to practical use. Appl. Biochem. Microbiol. 2008, 44, 453–456. [Google Scholar] [CrossRef]
- Wilson, C.R.; Luckman, G.A. Enhanced resistance to common scab of potato through somatic cell selection in cv. Iwa with the phytotoxin thaxtomin A. Plant Pathol. 2009, 58, 137–144. [Google Scholar] [CrossRef]
- Curir, P.; Guglieri, L. Fusaric Acid Production by Fusarium Oxysporum f.sp. lilii and its Role in the Lily Basal Rot Disease. Eur. J. Plant Pathol. 2000, 106, 849–856. [Google Scholar] [CrossRef]
- Companioni, B.; Arzola, M. Use of culture-derived Fusarium oxysporum f. sp. cubense, race 1 filtrates for rapid and non-destructive in vitro differentiation between resistant and susceptible clones of field-grown banana. Euphytica 2003, 130, 341–347. [Google Scholar] [CrossRef]
- Pandey, A.K.; Chandla, P. Herbicidal potential of secondary metabolites of some fungi against Lantana camara L. J. Mycol. Plant Pathol. 2002, 32, 100–102. [Google Scholar]
- Saxena, S.; Pandey, A.K. Microbial metabolites as eco-friendly agrochemicals for the next millennium. Appl. Microbiol. Biotechnol. 2001, 55, 395–403. [Google Scholar] [CrossRef]
- Kastanias, M.A.; Chrysayi-Tokousbalides, M. Herbicidal potential of pyrenophorol isolated from a Drechslera avenae pathotype. Pest Manag. Sci. 2000, 56, 227–232. [Google Scholar] [CrossRef]
- Evidente, A.; Andolfi, A. Stimulation of Orobanche ramosa seed germination by fusicoccin derivatives: A structure–activity relationship study. Phytochemistry 2006, 67, 19–26. [Google Scholar] [CrossRef]
- Evidente, A.; Lanzetta, R. Putaminoxin, a phytotoxic nonenolide from Phoma putaminum. Phytochemistry 1995, 40, 1637–1641. [Google Scholar] [CrossRef]
- Pedras, M.S.C.; Erosa-López, C.C. Phomalairdenone: A new host-selective phytotoxin from a virulent type of the blackleg fungus Phoma lingam. Bioorg. Med. Chem. Lett. 1999, 9, 3291–3294. [Google Scholar] [CrossRef]
- Rivero-Cruz, J.F.; Macías, M. A new phytotoxic nonenolide from Phoma herbarum. J. Nat. Prod. 2003, 66, 511–514. [Google Scholar] [CrossRef] [PubMed]
- Rivero-Cruz, J.F.; García-Aguirre, G. Conformational Behavior and Absolute Stereostructure of Two Phytotoxic Nonenolides from the Fungus Phoma herbarum. Tetrahedron 2000, 56, 5337–5344. [Google Scholar] [CrossRef]
- Ichihara, A.; Sawamura, S. Dihydrogladiolic Acid, another Phytotoxin from Phoma asparagi Sacc. Agric. Biol. Chem. 1985, 49, 1891–1892. [Google Scholar]
- Poluektova, E.; Tokarev, Y. Curvulin and Phaeosphaeride A from Paraphoma sp. VIZR 1.46 Isolated from Cirsium arvense as Potential Herbicides. Molecules 2018, 23, 2795. [Google Scholar] [CrossRef]
- Yoshida, S.; Hiradate, S. Colletotrichum dematium Produces Phytotoxins in Anthracnose Lesions of Mulberry Leaves. Phytopathology 2000, 90, 285–291. [Google Scholar] [CrossRef]
- Graupner, P.R.; Carr, A. The Macrocidins: Novel Cyclic Tetramic Acids with Herbicidal Activity Produced by Phoma macrostoma. J. Nat. Prod. 2003, 66, 1558–1561. [Google Scholar] [CrossRef]
- Boughalleb, N.; Mahjoub, M.E. In vitro Determination of Fusarium spp. Infection on Watermelon Seeds and their Localization. Plant Pathol. J. 2006, 5, 178–182. [Google Scholar] [CrossRef]
- Alam, S.S.; Bilton, J.N. Chickpea blight: Production of the phytotoxins solanapyrones A and C by Ascochyta rabiei. Phytochemistry 1989, 28, 2627–2630. [Google Scholar] [CrossRef]
- Mehta, Y.R.; Brogin, R.L. Phytotoxicity of a Culture Filtrate Produced by Stemphylium solani of Cotton. Plant Dis. 2000, 84, 838–842. [Google Scholar] [CrossRef] [PubMed]
- Li, Z.; Zhang, H. Characterization of phytotoxin and secreted proteins identifies of Lasiodiplodia theobromae, causes of peach gummosis. Fungal Biol. 2019, 123, 51–58. [Google Scholar] [CrossRef] [PubMed]
- Lazarovits, G. Purification and Partial Characterization of a Glycoprotein Toxin Produced by Cladosporium fulvum. Phytopathology 1979, 69, 1062–1068. [Google Scholar] [CrossRef]
- Cimmino, A.; Andolfi, A. Production of phytotoxins by Phoma exigua var. exigua, a potential mycoherbicide against perennial thistles. J. Agric. Food Chem. 2008, 56, 6304–6309. [Google Scholar] [CrossRef] [PubMed]
- Wang, Y.Z.; Miller, J.D. Effects of Fusarium graminearum Metabolites on Wheat Tissue in Relation to Fusarium Head Blight Resistance. J. Phytopathol. 1988, 122, 118–125. [Google Scholar] [CrossRef]
- Brooks, S.A. Sensitivity to a Phytotoxin from Rhizoctonia solani Correlates with Sheath Blight Susceptibility in Rice. Phytopathology 2007, 97, 1207–1212. [Google Scholar] [CrossRef]
- Clarke, C.R.; Kramer, C.G. Cultivar Resistance to Common Scab Disease of Potato Is Dependent on the Pathogen Species. Phytopathology 2019, 109, 1544–1554. [Google Scholar] [CrossRef]
- Wilson, C.R.; Tegg, R.S. Stable and Extreme Resistance to Common Scab of Potato Obtained Through Somatic Cell Selection. Phytopathology 2010, 100, 460–467. [Google Scholar] [CrossRef]
- Park, P.; Ikeda, K. Ultrastructural analysis of responses of host and fungal cells during plant infection. J. Gen. Plant Pathol. 2008, 74, 2–14. [Google Scholar] [CrossRef]
- Suzuki, T.; Shinogi, T. β-1,3-D-Glucan Transported from Golgi Apparatus of Japanese Pear Leaves is a Component of Extracellular Polysaccharides Accumulated after AK-toxin I Treatment. J. Gen. Plant Pathol. 2002, 68, 267–276. [Google Scholar] [CrossRef]
- Tsuge, T.; Harimoto, Y. Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata. FEMS Microbiol. Rev. 2013, 37, 44–66. [Google Scholar] [CrossRef] [PubMed]
- Zheng, L.; Lv, R. Isolation, Purification, and Biological Activity of a Phytotoxin Produced by Stemphylium solani. Plant Dis. 2010, 94, 1231–1237. [Google Scholar] [CrossRef] [PubMed]
- Chen, J.; Guo, Z. Research Status of Alfalfa Disease, Insect Pests and Weed. J. Grassl. Forage Sci. 2022, 1, 14. [Google Scholar]
- Da Matos, C.d.C.; Monteiro, L.C.P. Changes in soil microbial communities modulate interactions between maize and weeds. Plant Soil 2019, 440, 249–264. [Google Scholar] [CrossRef]
- Yang, C.; Tang, W. Weeds in the Alfalfa Field Decrease Rhizosphere Microbial Diversity and Association Networks in the North China Plain. Front. Microbiol. 2022, 13, 840774. [Google Scholar] [CrossRef] [PubMed]
- Raoofi, M.; Alebrahim, M.T. Efficiency of herbicides dose in mixture with cytogate for weed control in alfalfa (Medicago sativa L.). Appl. Ecol. Environ. Res. 2017, 15, 249–265. [Google Scholar] [CrossRef]
- Graupner, P.R.; Gerwick, B.C. Chlorosis Inducing Phytotoxic Metabolites: New Herbicides from Phoma macrostoma; American Chemical Society: Washington, DC, USA, 2006; Volume 927, pp. 37–47. [Google Scholar]
- Li, X.-J.; Gao, J.-M. Toxins from a symbiotic fungus, Leptographium qinlingensis associated with Dendroctonus armandi and their in vitro toxicities to Pinus armandi seedlings. Eur. J. Plant Pathol. 2012, 134, 239–247. [Google Scholar] [CrossRef]
- Dai, Z.B.; Wang, X. Secondary Metabolites and Their Bioactivities Produced by Paecilomyces. Molecules 2020, 25, 5077. [Google Scholar] [CrossRef]
- Musser, S.M.; Gay, M.L. Identification of a new series of fumonisins containing 3-hydroxypyridine. J. Nat. Prod. 1996, 59, 970–972. [Google Scholar] [CrossRef]
- Schwarz, M.; Köpcke, B. 3-Hydroxypropionic acid as a nematicidal principle in endophytic fungi. Phytochemistry 2004, 65, 2239–2245. [Google Scholar] [CrossRef]
S. No. | RT | Name of the Compound | Mol. Formula | MW | Peak Area | CAS | Structure |
---|---|---|---|---|---|---|---|
1 | 14.35 | 4-Hydroxyphenylethanol | C8H10O2 | 179 | 3.50% | 2380-91-8 | |
2 | 12.79 | 5-methylresorcinol | C7H8O2 | 253 | 1% | 504-15-4 | |
3 | 8.61 | 3-hydroxypyridine | C5H5NO | 152 | 0.90% | 109-00-2 | |
4 | 14.38 | 3-Hydroxypropionic acid | C3H6O3 | 103 | 2.80% | 503-66-2 |
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Dang, S.-Z.; Li, Y.-Z. The Characterization and the Biological Activity of Phytotoxin Produced by Paraphoma radicina. J. Fungi 2022, 8, 867. https://doi.org/10.3390/jof8080867
Dang S-Z, Li Y-Z. The Characterization and the Biological Activity of Phytotoxin Produced by Paraphoma radicina. Journal of Fungi. 2022; 8(8):867. https://doi.org/10.3390/jof8080867
Chicago/Turabian StyleDang, Shu-Zhong, and Yan-Zhong Li. 2022. "The Characterization and the Biological Activity of Phytotoxin Produced by Paraphoma radicina" Journal of Fungi 8, no. 8: 867. https://doi.org/10.3390/jof8080867