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J. Fungi 2017, 3(2), 17; doi:10.3390/jof3020017

Molecular Identification and Antifungal Susceptibility Patterns of Clinical Dermatophytes Following CLSI and EUCAST Guidelines

Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India
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Author to whom correspondence should be addressed.
Academic Editors: Ana Alastruey-Izquierdo and Stéphane Ranque
Received: 6 February 2017 / Revised: 20 March 2017 / Accepted: 20 March 2017 / Published: 23 March 2017
(This article belongs to the Special Issue Antifungal Susceptibility Testing)
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Abstract

Dermatophytes are associated with superficial infections in humans worldwide. The aim of the present study was to determine the species distribution and susceptibility patterns of clinical dermatophytes. Samples received for routine mycological processing from 124 suspected cases attending a dermatologic clinic in a tertiary care hospital were included in the study. On direct microscopy, 74.1% (92/124) were positive and 53.2% (66/124) grew on culture. The isolates were comprised of Trichophyton interdigitale (56%) followed by Trichophyton tonsurans (25.7%), Trichophyton rubrum (7.5%), Trichophyton violaceum (4.5%), Microsporum gypseum (4.5%), and Trichophyton verrucosum (1.5%). Conventional mycological identification was concordant with ITS sequencing except for T. mentagrophytes. High minimum inhibitory concentration (MIC) values (geometric mean, >1 µg/mL) were observed for T. tonsurans and T. rubrum to terbinafine and griseofulvin. This study highlights the shift in epidemiology from T. rubrum to T. interdigitale. It also raises a concern of high MICs of terbinafine and griseofulvin among our isolates. Surveillance of antifungal susceptibility patterns can provide clinicians with local MIC data that can further aid in guiding better management in relapse cases of dermatomycosis. View Full-Text
Keywords: dermatophytes; internal transcribed spacer (ITS) sequencing; antifungal susceptibility; CLSI M38-A2; EUCAST E-Def 9.2 revision; India dermatophytes; internal transcribed spacer (ITS) sequencing; antifungal susceptibility; CLSI M38-A2; EUCAST E-Def 9.2 revision; India
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Dabas, Y.; Xess, I.; Singh, G.; Pandey, M.; Meena, S. Molecular Identification and Antifungal Susceptibility Patterns of Clinical Dermatophytes Following CLSI and EUCAST Guidelines. J. Fungi 2017, 3, 17.

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