Purification and Characterisation of Badger IgA and Its Detection in the Context of Tuberculosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Purification of sIgA from Badger Bile
2.2. Generation of Monoclonal Antibodies to Badger IgA
2.3. Specificity of Hybridomas for Badger IgA and IgG in a Direct ELISA
2.4. Source of Samples
2.5. Detection of MPB83 Specific-IgA in Serum and Tracheal Aspirate by ELISA
2.6. Immunohistochemistry Staining
3. Results
3.1. Purification and Western Blot, of Badger sIgA
3.2. Recognition by IgA Monoclonal Antibodies of Purified IgA and IgG
3.3. Recognition of Recombinant MPB83 by IgA in Serum and Tracheal Aspirate
3.4. Immunohistochemical Staining with Peroxidase Labelled IgA Monoclonal
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species. | Source | H-Chain (kDa) | L-Chain (kDa) | SC (kDa) | Reference |
---|---|---|---|---|---|
Badger | Bile | 46 | 27 | 66 | This work |
Rat | Bile Milk | 50–58 NK 1 | 25–28 NK | 66–80 66–71 | [22] |
Asian elephant | Milk | 55–60 | NK | 68–82 | [23,24] |
Guinea pig | Milk | 52 | 24 | 72–88 | [25,26] |
Human | Milk Colostrum | 57–64 | 25–28 | 73–90 76 | [22,27,28] |
Pig | Milk | 55–57 | 23 | 72–90 | [29,30] |
W.I. manatee | Milk | 55–57 | NK | 70–86 | [24] |
Baboon | Colostrum | 45–66 | 30 | 66–97 | [31] |
Rabbit | Colostrum | 62–67 | 23 | 64 | [26,32] |
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Dalley, D.; Lesellier, S.; Salguero, F.J.; Chambers, M.A. Purification and Characterisation of Badger IgA and Its Detection in the Context of Tuberculosis. Vet. Sci. 2019, 6, 89. https://doi.org/10.3390/vetsci6040089
Dalley D, Lesellier S, Salguero FJ, Chambers MA. Purification and Characterisation of Badger IgA and Its Detection in the Context of Tuberculosis. Veterinary Sciences. 2019; 6(4):89. https://doi.org/10.3390/vetsci6040089
Chicago/Turabian StyleDalley, Deanna, Sandrine Lesellier, Francisco J. Salguero, and Mark A. Chambers. 2019. "Purification and Characterisation of Badger IgA and Its Detection in the Context of Tuberculosis" Veterinary Sciences 6, no. 4: 89. https://doi.org/10.3390/vetsci6040089