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Vet. Sci. 2017, 4(2), 31; doi:10.3390/vetsci4020031

Detection and Characterization of Histamine-Producing Strains of Photobacterium damselae subsp. damselae Isolated from Mullets

1
Dipartimento di Scienze Mediche Veterinarie, Università degli Studi di Bologna, Alma Mater Studiorum, via Tolara di Sopra 50, Ozzano Emilia 40064, Italy
2
S.S. Laboratorio Specialistico Ittiopatologia, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Torino 10154, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Chrissanthy Papadopoulou, Vangelis Economou and Hercules Sakkas
Received: 13 April 2017 / Revised: 8 June 2017 / Accepted: 13 June 2017 / Published: 20 June 2017
(This article belongs to the Special Issue Food and Waterborne Infections in Animals and Humans)
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Abstract

Photobacterium damselae subsp. damselae (Pdd) is considered to be an emerging pathogen of marine fish and has also been implicated in cases of histamine food poisoning. In this study, eight strains isolated from mullets of the genera Mugil and Liza captured in the Ligurian Sea were characterized, and a method to detect histamine-producing Pdd from fish samples was developed. The histamine-producing potential of the strains was evaluated in culture media (TSB+) using a histamine biosensor. Subsequently, two strains were used to contaminate mackerel fillets (4 or 40 CFU/g), simulating a cross-contamination on the selling fish stalls. Sample homogenates were enriched in TSB+. The cultures were then inoculated on thiosulfate-citrate-bile salts-sucrose agar (TCBS) and the dark green colonies were cultured on Niven agar. The violet isolates were characterized using specific biochemical and PCR based tests. All Pdd strains were histamine producers, yielding concentration varying from 167 and 8977 µg/mL in TSB+ cultures incubated at 30 °C for 24 h. Pdd colonies were detected from the inoculated mackerel samples and their histidine decarboxylase gene was amplified using species-specific primer pairs designed for this study. The results indicate that mullets can be source of Pdd and the fish retailers needs to evaluate the risk posed by cross-contamination on the selling fish stalls. View Full-Text
Keywords: Photobacterium damselae subsp. damselae; histidine decarboxylase; cross contamination; mullets; histamine biosensor Photobacterium damselae subsp. damselae; histidine decarboxylase; cross contamination; mullets; histamine biosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Trevisani, M.; Mancusi, R.; Cecchini, M.; Costanza, C.; Prearo, M. Detection and Characterization of Histamine-Producing Strains of Photobacterium damselae subsp. damselae Isolated from Mullets. Vet. Sci. 2017, 4, 31.

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