Next Article in Journal
Characterization of Haptoglobin Isotype in Milk of Mastitis-Affected Cows
Next Article in Special Issue
Regarding Tick-Borne Relapsing Fever in the Americas; Some Historical Aspects of a Forgotten Disease in Colombia
Previous Article in Journal / Special Issue
Deviant Behavior: Tick-Borne Pathogens and Inflammasome Signaling
Article Menu

Export Article

Open AccessArticle
Vet. Sci. 2016, 3(4), 28; doi:10.3390/vetsci3040028

Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems

1
The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UK
2
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Ulrike Munderloh
Received: 28 July 2016 / Revised: 9 September 2016 / Accepted: 22 September 2016 / Published: 29 September 2016
(This article belongs to the Special Issue Comparative Studies in Tick-Borne Diseases in Animals and Humans)
View Full-Text   |   Download PDF [7739 KB, uploaded 29 September 2016]   |  

Abstract

Ticks are vectors and reservoirs of many arboviruses pathogenic for humans or domestic animals; in addition, during bloodfeeding they can acquire and harbour pathogenic arboviruses normally transmitted by other arthropods such as mosquitoes. Tick cell and organ cultures provide convenient tools for propagation and study of arboviruses, both tick-borne and insect-borne, enabling elucidation of virus-tick cell interaction and yielding insight into the mechanisms behind vector competence and reservoir potential for different arbovirus species. The mosquito-borne zoonotic alphavirus Semliki Forest virus (SFV), which replicates well in tick cells, has been isolated from Rhipicephalus, Hyalomma, and Amblyomma spp. ticks removed from mammalian hosts in East Africa; however nothing is known about any possible role of ticks in SFV epidemiology. Here we present a light and electron microscopic study of SFV infecting cell lines and organ cultures derived from African Rhipicephalus spp. ticks. As well as demonstrating the applicability of these culture systems for studying virus-vector interactions, we provide preliminary evidence to support the hypothesis that SFV is not normally transmitted by ticks because the virus does not infect midgut cells. View Full-Text
Keywords: tick cell line; tick organ culture; arbovirus; Semliki Forest virus; reporter gene; microscopy tick cell line; tick organ culture; arbovirus; Semliki Forest virus; reporter gene; microscopy
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Bell-Sakyi, L.; Weisheit, S.; Rückert, C.; Barry, G.; Fazakerley, J.; Fragkoudis, R. Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems. Vet. Sci. 2016, 3, 28.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Vet. Sci. EISSN 2306-7381 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top