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Open AccessCommunication
Vet. Sci. 2015, 2(2), 84-96; doi:10.3390/vetsci2020084

Intranuclear Inclusions in Renal Tubular Epithelium in Immunodeficient Mice Stain with Antibodies for Bovine Papillomavirus Type 1 L1 Protein

1
Cerberus Sciences, Unit 3, 49 Holland Street, Thebarton, SA 5031, Australia
2
School of Veterinary and Life Sciences, Murdoch University, South Street, Murdoch, WA 6150, Australia
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editors: Margarethe Hoenig and Duncan C. Ferguson
Received: 4 May 2015 / Revised: 1 June 2015 / Accepted: 5 June 2015 / Published: 11 June 2015
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Abstract

The kidneys from six immunodeficient mice examined by Cerberus Sciences and the Animal Resources Centre, displayed karyomegaly with pale eosinophilic, intranuclear inclusions upon histopathological examination. Electron microscopy performed on kidney tissue from 5/6 mice demonstrated margination of the chromatin in large nuclei. Laboratory tests were used to detect nucleic acid of papillomaviruses, polyomaviruses, circoviruses and anelloviruses (4/6 mice), a specific PCR was used to detect murine polyomavirus (1/6), and a panel of serological tests was used to detect seroconversion to major murine pathogens (1/6). All molecular and serological tests were negative. Immunohistochemistry using polyclonal anti-bovine papillomavirus type 1 (BPV-1) L1 antibody, Camvir monoclonal anti-papillomavirus antibody (directed against the seven amino acids GFGAMDF found in human papillomavirus (HPV) 16 L1 protein), a commercially available mixture of two monoclonal antibodies, anti-BPV-1 L1/1H8 + Camvir antibodies, and a monoclonal anti-Hsc70 antibody revealed specific, positive staining of murine renal tubular epithelial intranuclear inclusions in 6/6 mice using the anti-BPV-1 L1 containing antibodies only. Methyl pyronin green, PAS and Feulgen histochemical reactions revealed that the intranuclear inclusions did not consist of RNA, DNA or carbohydrate. An immunohistochemical method now exists that can be used to confirm and evaluate suspected cases of murine inclusion body nephropathy. View Full-Text
Keywords: mouse; intranuclear inclusions; renal tubular epithelium; bovine papillomavirus type 1 L1 protein mouse; intranuclear inclusions; renal tubular epithelium; bovine papillomavirus type 1 L1 protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

McInnes, E.; Bennett, M.; O'Hara, M.; Rasmussen, L.; Fung, P.; Nicholls, P.; Slaven, M.; Stevenson, R. Intranuclear Inclusions in Renal Tubular Epithelium in Immunodeficient Mice Stain with Antibodies for Bovine Papillomavirus Type 1 L1 Protein. Vet. Sci. 2015, 2, 84-96.

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