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Beverages 2016, 2(2), 8; doi:10.3390/beverages2020008

Effect of Different Conditions of Growth and Storage on the Cell Counts of Two Lactic Acid Bacteria after Spray Drying in Orange Juice

Universidade Católica Portuguesa, CBQF-Centro de Biotecnologia e Química Fina–Laboratório Associado, Escola Superior de Biotecnologia, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal
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Academic Editor: Antonio Bevilacqua
Received: 15 February 2016 / Revised: 17 March 2016 / Accepted: 17 March 2016 / Published: 29 March 2016
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Abstract

Consumers increasingly require innovative food products with health benefits. Thus, a dried orange juice incorporating probiotics could be a novel challenge. In this context, we investigated whether different sugars added to the culture media used for growth of two lactic acid bacteria contributed to their protection during spray drying in orange juice and subsequent storage under different conditions of temperature, light exposure and water activity. Cell viability during passage through simulated gastro-intestinal conditions was also investigated. Cells grown in culture medium containing fructose resulted in the worst survival rates during storage. High survival was observed for cells grown in the presence of lactose, followed by glucose. The survival of dried bacteria was enhanced at 4 °C, water activity of 0.03 and absence of daylight. For cells grown in standard culture medium and after 12 months of storage at 4 °C in orange juice powder (about 109 cfu/mL), there was a reduction of approximately 2 log-units for both lactic acid bacteria after gastro-intestinal tract passage simulation. Using the conditions of growth and storage investigated, it is possible to improve the survival rate of lactic acid bacteria and produce an orange juice powder with probiotic characteristics with shelf life of at least 12 months. View Full-Text
Keywords: gastro-intestinal tract; Lactobacillus plantarum 299v; Pediococcus acidilactici HA-6111-2; probiotic juice; sugars gastro-intestinal tract; Lactobacillus plantarum 299v; Pediococcus acidilactici HA-6111-2; probiotic juice; sugars
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Barbosa, J.; Borges, S.; Teixeira, P. Effect of Different Conditions of Growth and Storage on the Cell Counts of Two Lactic Acid Bacteria after Spray Drying in Orange Juice. Beverages 2016, 2, 8.

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