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Bioengineering 2017, 4(2), 56; doi:10.3390/bioengineering4020056

Three-Dimensional Culture Model of Skeletal Muscle Tissue with Atrophy Induced by Dexamethasone

1
Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan
2
Innovative Research Center for Preventive Medical Engineering, Nagoya University, Nagoya 464-8601, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Hyun Jung Kim
Received: 12 April 2017 / Revised: 12 June 2017 / Accepted: 12 June 2017 / Published: 15 June 2017
(This article belongs to the Special Issue Human Organs-on-Chips for In Vitro Disease Models)
View Full-Text   |   Download PDF [1857 KB, uploaded 15 June 2017]   |  

Abstract

Drug screening systems for muscle atrophy based on the contractile force of cultured skeletal muscle tissues are required for the development of preventive or therapeutic drugs for atrophy. This study aims to develop a muscle atrophy model by inducing atrophy in normal muscle tissues constructed on microdevices capable of measuring the contractile force and to verify if this model is suitable for drug screening using the contractile force as an index. Tissue engineered skeletal muscles containing striated myotubes were prepared on the microdevices for the study. The addition of 100 µM dexamethasone (Dex), which is used as a muscle atrophy inducer, for 24 h reduced the contractile force significantly. An increase in the expression of Atrogin-1 and MuRF-1 in the tissues treated with Dex was established. A decrease in the number of striated myotubes was also observed in the tissues treated with Dex. Treatment with 8 ng/mL Insulin-like Growth Factor (IGF-I) for 24 h significantly increased the contractile force of the Dex-induced atrophic tissues. The same treatment, though, had no impact on the force of the normal tissues. Thus, it is envisaged that the atrophic skeletal muscle tissues induced by Dex can be used for drug screening against atrophy. View Full-Text
Keywords: cell-based assay; disease model; drug screening; contractile force; microdevice cell-based assay; disease model; drug screening; contractile force; microdevice
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Shimizu, K.; Genma, R.; Gotou, Y.; Nagasaka, S.; Honda, H. Three-Dimensional Culture Model of Skeletal Muscle Tissue with Atrophy Induced by Dexamethasone. Bioengineering 2017, 4, 56.

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