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Open AccessCommunication
Bioengineering 2016, 3(4), 27; doi:10.3390/bioengineering3040027

A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling

Clinical Pharmacology and Therapeutics Unit, Department of Medicine, University of Melbourne, Austin Health, Heidelberg Victoria, Heidelberg 3084, Australia
Running Title: Juxtacellular Neuronal Labelling Pulse Source.
Academic Editor: Christoph Herwig
Received: 7 September 2016 / Revised: 22 September 2016 / Accepted: 17 October 2016 / Published: 19 October 2016
View Full-Text   |   Download PDF [1755 KB, uploaded 19 October 2016]   |  

Abstract

Juxtacellular neuronal labelling is a method which allows neurophysiologists to fill physiologically-identified neurons with small positively-charged marker molecules. Labelled neurons are identified by histochemical processing of brain sections along with immunohistochemical identification of neuropeptides, neurotransmitters, neurotransmitter transporters or biosynthetic enzymes. A microcontroller-based pulser circuit and associated BASIC software script is described for incorporation into the design of a commercially-available intracellular electrometer for use in juxtacellular neuronal labelling. Printed circuit board construction has been used for reliability and reproducibility. The current design obviates the need for a separate digital pulse source and simplifies the juxtacellular neuronal labelling procedure. View Full-Text
Keywords: juxtacellular; Picaxe; neurophysiology; neuron; microcontroller juxtacellular; Picaxe; neurophysiology; neuron; microcontroller
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Verberne, A.J.M. A Simple Picaxe Microcontroller Pulse Source for Juxtacellular Neuronal Labelling. Bioengineering 2016, 3, 27.

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