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Bioengineering 2016, 3(3), 17; doi:10.3390/bioengineering3030017

Optimization and Characterization of Chitosan Enzymolysis by Pepsin

Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, China
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Academic Editor: Christoph Herwig
Received: 11 March 2016 / Revised: 9 May 2016 / Accepted: 28 June 2016 / Published: 1 July 2016
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Abstract

Pepsin was used to effectively degrade chitosan in order to make it more useful in biotechnological applications. The optimal conditions of enzymolysis were investigated on the basis of the response surface methodology (RSM). The structure of the degraded product was characterized by degree of depolymerization (DD), viscosity, molecular weight, FTIR, UV-VIS, SEM and polydispersity index analyses. The mechanism of chitosan degradation was correlated with cleavage of the glycosidic bond, whereby the chain of chitosan macromolecules was broken into smaller units, resulting in decreasing viscosity. The enzymolysis by pepsin was therefore a potentially applicable technique for the production of low molecular chitosan. Additionally, the substrate degradation kinetics of chitosan were also studied over a range of initial chitosan concentrations (3.0~18.0 g/L) in order to study the characteristics of chitosan degradation. The dependence of the rate of chitosan degradation on the concentration of the chitosan can be described by Haldane’s model. In this model, the initial chitosan concentration above which the pepsin undergoes inhibition is inferred theoretically to be about 10.5 g/L. View Full-Text
Keywords: chitosan; enzymolysis; pepsin; response surface methodology; inhibition; kinetic chitosan; enzymolysis; pepsin; response surface methodology; inhibition; kinetic
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gohi, B.F.C.A.; Zeng, H.-Y.; Pan, A.D. Optimization and Characterization of Chitosan Enzymolysis by Pepsin. Bioengineering 2016, 3, 17.

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