Next Article in Journal / Special Issue
Improved Anaerobic Fermentation of Wheat Straw by Alkaline Pre-Treatment and Addition of Alkali-Tolerant Microorganisms
Previous Article in Journal / Special Issue
Microbial Community Shifts during Biogas Production from Biowaste and/or Propionate
Article Menu

Export Article

Open AccessArticle
Bioengineering 2015, 2(2), 54-65; doi:10.3390/bioengineering2020054

Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts

1
Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands
2
Sub-Department of Environmental Biotechnology, Wageningen University, 6700 AA Wageningen, The Netherlands
3
Section Sanitary Engineering, Department of Water Management, Faculty of Civil Engineering and Geosciences, Delft University of Technology, 2600 GA Delft, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Sabine Kleinsteuber
Received: 29 January 2015 / Accepted: 13 March 2015 / Published: 25 March 2015
(This article belongs to the Special Issue Microbial Ecology of Anaerobic Digestion)
View Full-Text   |   Download PDF [781 KB, uploaded 25 March 2015]   |  

Abstract

Humic compounds are inhibitory to the anaerobic hydrolysis of cellulosic biomass. In this study, the impact of salt addition to mitigate the inhibitory effects of humic compounds was investigated. The experiment was conducted using batch tests to monitor the anaerobic hydrolysis of cellulose in the presence of humic acid. Sodium, potassium, calcium, magnesium and iron salts were tested separately for their efficiency to mitigate humic acid inhibition. All experiments were done under mesophilic conditions (30 °C) and at pH 7. Methane production was monitored online, using the Automatic Methane Potential Test System. Methane production, soluble chemical oxygen demand and volatile fatty acid content of the samples were measured to calculate the hydrolysis efficiencies. Addition of magnesium, calcium and iron salts clearly mitigated the inhibitory effects of humic acid and hydrolysis efficiencies reached up to 75%, 65% and 72%, respectively, which were similar to control experiments. Conversely, potassium and sodium salts addition did not mitigate the inhibition and hydrolysis efficiencies were found to be less than 40%. Mitigation of humic acid inhibition via salt addition was also validated by inductively coupled plasma atomic emission spectroscopy analyses, which showed the binding capacity of different cations to humic acid. View Full-Text
Keywords: cellulose; anaerobic digestion; humic acid; hydrolysis; inhibition; mitigation; salt addition; cations cellulose; anaerobic digestion; humic acid; hydrolysis; inhibition; mitigation; salt addition; cations
Figures

Figure 1a

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Azman, S.; Khadem, A.F.; Zeeman, G.; van Lier, J.B.; Plugge, C.M. Mitigation of Humic Acid Inhibition in Anaerobic Digestion of Cellulose by Addition of Various Salts. Bioengineering 2015, 2, 54-65.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Bioengineering EISSN 2306-5354 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top