Developing a Commercial Antimicrobial Active Packaging System of Ground Beef Based on “Tsipouro” Alcoholic Distillate
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antimicrobial Compound
2.2. Preparation and Storage of Ground Beef
2.3. Headspace Gas Analysis
2.4. Microbiological Analysis
2.5. PH Measurement
2.6. Model Development of B. thermosphacta and LAB Growth
2.7. Colour Measurement
2.8. Model Development of a* Value
2.9. Validation of the Developed Models for Microbial Growth and Colour Deterioration
2.10. Evaluation of Models Performance
2.11. Sensory Evaluation
2.12. SPME/GC-Flame Ionization Detector Analysis
2.13. Statistical Analysis
3. Results and Discussion
3.1. Headspace Gas Analysis
3.2. Kinetics of Microbiological Data and Model Development/Validation
3.3. Colour Measurements and Model Development/Validation of a* Value
3.4. Sensory Evaluation
3.5. SPME/GC-Flame Ionization Detector Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Microorganism/ Treatment | Temperature (°C) | Ν0 (log CFU/g) 1 | Νmax (log CFU/g) 1 | µmax (day−1) 1 | λ (days) |
---|---|---|---|---|---|
B. thermosphacta | |||||
Control | 0 | 3.91 ± 0.96 A | 7.00 ± 0.56 A | 0.36 ± 0.14 A | N.A. 2 |
4 | 4.75 ± 0.75 AB | 8.32 ± 0.31 C | 0.65 ± 0.18 B | N.A. | |
8 | 4.18 ± 1.27 AB | 7.55 ± 0.34 AB | 1.26 ± 0.06 C | N.A. | |
12 | 5.82 ± 0.21 B | 8.04 ± 0.24 BC | 1.46 ± 0.06 C | N.A. | |
“Tsipouro” | 0 | 4.10 ± 0.89 A | 6.38 ± 0.45 A | 0.14 ± 0.04 A* | N.A. |
4 | 5.06 ± 1.09 A | 7.27 ± 0.55 AB* | 0.37 ± 0.16 A* | N.A. | |
8 | 4.14 ± 1.08 A | 6.66 ± 0.50 A* | 0.72 ± 0.26 B* | N.A. | |
12 | 5.85 ± 0.17 A | 7.93 ± 0.20 B | 1.15 ± 0.11 C | N.A. | |
LAB | |||||
Control | 0 | 2.92 ± 0.31 A | 6.51 ± 0.39 A | 0.28 ± 0.02 A | N.A. |
4 | 3.62 ± 0.53 AB | 6.55 ± 0.14 A | 0.45 ± 0.19 A | N.A. | |
8 | 4.44 ± 1.47 B | 6.85 ± 0.65 A | 0.87 ± 0.04 B | N.A. | |
12 | 3.89 ± 0.34 AB | 6.68 ± 0.06 A | 1.64 ± 0.07 C | N.A. | |
“Tsipouro” | 0 | 3.11 ± 0.21 A | 6.74 ± 0.77 A | 0.23 ± 0.05 A | N.A. |
4 | 3.90 ± 0.95 A | 6.13 ± 1.11 A | 0.17 ± 0.06 A* | N.A. | |
8 | 4.31 ± 1.36 A | 6.75 ± 0.67 A | 0.65 ± 0.16 B* | N.A. | |
12 | 3.92 ± 0.35 A | 6.61 ± 0.19 A | 1.46 ± 0.06 C* | N.A. |
Parameters and Statistical Indices | B. thermosphacta | LAB | ||||||
---|---|---|---|---|---|---|---|---|
Control | “Tsipouro” | Control | “Tsipouro” | |||||
Estimate | p-Value | Estimate | p-Value | Estimate | p-Value | Estimate | p-Value | |
Square Root Model | ||||||||
b | 0.054 ± 0.005 | 0.000 | 0.059 ± 0.006 | 0.000 | 0.059 ± 0.008 | 0.000 | 0.063 ± 0.009 | 0.000 |
T0 (°C) | −11.3 ± 1.6 | 0.000 | −6.2 ± 1.2 | 0.000 | −7.8 ± 1.3 | 0.000 | −4.9 ± 1.6 | 0.000 |
R2 | 0.865 | 0.782 | 0.805 | 0.760 | ||||
RMSE | 0.017 | 0.016 | 0.019 | 0.029 | ||||
Arrhenius Model | ||||||||
Ea (kJ/mol) | 90.2 ± 12.2 | 0.000 | 87.2 ± 17.1 | 0.002 | 92.8 ± 10.4 | 0.000 | 124.8 ± 15.0 | 0.000 |
ln kref (day−1) | −0.586 ± 0.092 | 0.001 | −1.167 ± 0.129 | 0.000 | −0.833 ± 0.081 | 0.000 | −1.448 ± 0.113 | 0.000 |
R2 | 0.901 | 0.813 | 0.859 | 0.831 | ||||
RMSE | 0.057 | 0.111 | 0.082 | 0.173 |
Sampling Side | Treatment | Temperature (°C) | Kcolour (day−1) | R2 |
---|---|---|---|---|
Surface | Control | 0 | −0.852 ± 0.095 A | 0.976 |
4 | −1.079 ± 0.028 A | 0.949 | ||
8 | −2.060 ± 0.093 B | 0.937 | ||
12 | −5.322 ± 0.032 C | 0.971 | ||
“Tsipouro” | 0 | −0.343 ± 0.004 A* | 0.769 | |
4 | −0.737 ± 0.057 AB* | 0.961 | ||
8 | −0.838 ± 0.047 AB* | 0.888 | ||
12 | −0.953 ± 0.284 B* | 0.887 | ||
Bottom | Control | 0 | −0.472 ± 0.074 A | 0.846 |
4 | −1.027 ± 0.021 A | 0.972 | ||
8 | −1.039 ± 0.153 A | 0.869 | ||
12 | −2.966 ± 0.532 B | 0.906 | ||
“Tsipouro” | 0 | −0.242 ± 0.031 A* | 0.775 | |
4 | −0.578 ± 0.028 B* | 0.901 | ||
8 | −0.823 ± 0.045 C* | 0.913 | ||
12 | −1.261 ± 0.088 D* | 0.837 |
Parameters and Statistical Indices | Control | “Tsipouro” | ||
---|---|---|---|---|
Estimated Value | p-Value | Estimated Value | p-Value | |
Surface | ||||
Ea (kJ/mol) | 99.2 ± 11.3 | 0.000 | 63.6 ± 10.6 | 0.002 |
ln kref (day−1) | 0.279 ± 0.086 | 0.017 | −0.532 ± 0.070 | 0.001 |
R2 | 0.927 | 0.878 | ||
RMSE | 0.049 | 0.032 | ||
Bottom | ||||
Ea (kJ/mol) | 89.0 ± 14.1 | 0.001 | 86.1 ± 8.0 | 0.000 |
ln kref (day−1) | −0.172 ± 0.106 | 0.156 | −0.743 ± 0.061 | 0.000 |
R2 | 0.870 | 0.950 | ||
RMSE | 0.076 | 0.025 |
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Kapetanakou, A.E.; Pateraki, G.-L.; Skandamis, P.N. Developing a Commercial Antimicrobial Active Packaging System of Ground Beef Based on “Tsipouro” Alcoholic Distillate. Foods 2020, 9, 1171. https://doi.org/10.3390/foods9091171
Kapetanakou AE, Pateraki G-L, Skandamis PN. Developing a Commercial Antimicrobial Active Packaging System of Ground Beef Based on “Tsipouro” Alcoholic Distillate. Foods. 2020; 9(9):1171. https://doi.org/10.3390/foods9091171
Chicago/Turabian StyleKapetanakou, Anastasia E., Georgia-Lito Pateraki, and Panagiotis N. Skandamis. 2020. "Developing a Commercial Antimicrobial Active Packaging System of Ground Beef Based on “Tsipouro” Alcoholic Distillate" Foods 9, no. 9: 1171. https://doi.org/10.3390/foods9091171