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Foods 2018, 7(5), 66; https://doi.org/10.3390/foods7050066

HPLC-ED Analysis of Phenolic Compounds in Three Bosnian Crataegus Species

1
Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and Herzegovina
2
Department of Forest Ecology, Faculty of Forestry, University of Sarajevo, Zagrebačka 20, 71 000 Sarajevo, Bosnia and Herzegovina
*
Author to whom correspondence should be addressed.
Received: 20 March 2018 / Revised: 18 April 2018 / Accepted: 19 April 2018 / Published: 24 April 2018
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Abstract

The aim of this work was the qualitative and quantitative determination of selected phenolic compounds in three Crataegus species grown in Bosnia. Crataegus plants are consumed for medicinal purposes and as foodstuff in the form of canned fruit, jam, jelly, tea, and wine. Two samples of plant material, dry leaves with flowers, and berries of three Crataegus species—Crataegus rhipidophylla Gand., Crataegus x subsphaericea Gand., and Crataegus x macrocarpa Hegetschw.—were analyzed. Twelve ethanolic extracts were isolated from the selected plant material using Soxhlet and ultrasound extraction, respectively. Soxhlet extraction proved to be more effective than ultrasound extraction. A simple and sensitive method, high-performance liquid chromatography with electrochemical detection, HPLC-ED, was used for the simultaneous determination of phenolic acids and flavonoids in Crataegus species. The content of gallic acid in the extracts ranged from 0.001 to 0.082 mg/g dry weight (DW), chlorogenic acid from 0.19 to 8.70 mg/g DW, and rutin from 0.03 to 13.49 mg/g DW. Two flavonoids, vitexin and hyperoside, commonly found in chemotaxonomic investigations of Crataegus species, were not detected in the examined extracts. In general, leaves with flowers samples are richer in gallic acid and rutin, whereas the berries samples are richer in chlorogenic acid. Distinct similarities were found in the relative distribution of gallic acid among the three species. Extracts of C. x macrocarpa had the highest content of all detected compounds, while significant differences were found in rutin content, depending on the plant organ. To the best of our knowledge, this is the first study reporting content of phenolic compounds in Crataegus rhipidophylla Gand., Crataegus x subsphaericea, and Crataegus x macrocarpa from Bosnia. View Full-Text
Keywords: Crataegus; Soxhlet; ultrasound extraction; flavonoids; phenolic acids; HPLC-ED Crataegus; Soxhlet; ultrasound extraction; flavonoids; phenolic acids; HPLC-ED
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Čulum, D.; Čopra-Janićijević, A.; Vidic, D.; Klepo, L.; Tahirović, A.; Bašić, N.; Maksimović, M. HPLC-ED Analysis of Phenolic Compounds in Three Bosnian Crataegus Species. Foods 2018, 7, 66.

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