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Foods 2017, 6(11), 101; https://doi.org/10.3390/foods6110101

Immunodetection of Porcine Red Blood Cell Containing Food Ingredients Using a Porcine-Hemoglobin-Specific Monoclonal Antibody

Department of Nutrition, Food and Exercise Sciences, 420 Sandels Building, Florida State University, Tallahassee, FL 32306-1493, USA
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Received: 14 October 2017 / Revised: 10 November 2017 / Accepted: 13 November 2017 / Published: 20 November 2017
(This article belongs to the Special Issue Food Proteins and Bioactive Peptides)
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Abstract

Monoclonal antibody (mAb) 24C12-E7 has been found to bind to a 12 kDa antigenic protein in the red blood cell (RBC) of porcine blood. The purpose of this study was to determine the identity of this 12 kDa protein and consequently examine its potential as a marker for monitoring porcine RBC-containing ingredients (PRBCIs) in foods. Proteomic techniques identified the 12 kDa antigenic protein to be a monomer of the tetrameric hemoglobin molecule. Further heat-processing of spray-dried PRBCIs diminishes its detectability. Whereas mAb 24C12-E7-based indirect enzyme-linked immunosorbent assay (iELISA) could detect 1% (v/v) or less of PRBCIs in raw and cooked ground meats (beef, pork and chicken), the detection limits were 3 to 30 times higher for spiked cooked beef and pork. The assay is effective for monitoring the presence of PRBCIs in foods to protect the billions of people that avoid consuming blood. In situations where these PRBCIs are present as ingredients in foods that have undergone further heat processing, the assay, however, may not be as sensitive depending on the types of sample matrix, types of PRBCIs and the level of inclusion. View Full-Text
Keywords: monoclonal antibody; porcine blood; ingredients; red blood cells; hemoglobin; ELISA monoclonal antibody; porcine blood; ingredients; red blood cells; hemoglobin; ELISA
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ofori, J.A.; Hsieh, Y.-H.P. Immunodetection of Porcine Red Blood Cell Containing Food Ingredients Using a Porcine-Hemoglobin-Specific Monoclonal Antibody. Foods 2017, 6, 101.

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