Next Article in Journal
Comparative Evaluation of Diagnostic Tools for Oxidative Deterioration of Polyunsaturated Fatty Acid-Enriched Infant Formulas during Storage
Previous Article in Journal
Extraction and Characterization of Highly Gelling Low Methoxy Pectin from Cashew Apple Pomace
Article Menu

Export Article

Open AccessArticle
Foods 2014, 3(1), 13-29; doi:10.3390/foods3010013

Gluten Detection and Speciation by Liquid Chromatography Mass Spectrometry (LC-MS/MS)

AB SCIEX, Pheonix House, Centre Park, Warrington, WA1 1RX, UK
Received: 12 October 2013 / Revised: 12 December 2013 / Accepted: 18 December 2013 / Published: 23 December 2013
View Full-Text   |   Download PDF [465 KB, uploaded 23 December 2013]   |  


Liquid chromatography tandem mass spectrometry (LC-MS/MS) has been used historically in proteomics research for over 20 years. However, until recently LC-MS/MS has only been routinely used in food testing for small molecule contaminant detection, for example pesticide and veterinary residue detection, and not as a replacement of microbiological food testing methods, specifically allergen analysis. Over the last couple of years, articles have started to be published which describe the detection of allergens by LC-MS/MS. In this article we will describe how LC-MS/MS can be applied in the area of gluten detection and how it can be used to specifically differentiate the species of gluten used in food, where specific markers for each variety of gluten can be simultaneously acquired and detected at the same time. The article will discuss the effect of variety on the peptide response observed from different wheat grain varieties and will describe the sample preparation protocol which is essential for generating the peptide markers used for speciation. View Full-Text
Keywords: gluten; speciation; LC-MS/MS gluten; speciation; LC-MS/MS

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lock, S. Gluten Detection and Speciation by Liquid Chromatography Mass Spectrometry (LC-MS/MS). Foods 2014, 3, 13-29.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Foods EISSN 2304-8158 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top