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Dent. J. 2017, 5(1), 7; doi:10.3390/dj5010007

An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations

1
Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, Japan
2
Division of Implantology, Niigata University Medical and Dental Hospital, Niigata 951-8514, Japan
3
Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
4
Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan
5
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Louis M. Lin
Received: 21 November 2016 / Revised: 16 December 2016 / Accepted: 6 January 2017 / Published: 12 January 2017
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Abstract

Platelet concentrates should be quality-assured of purity and identity prior to clinical use. Unlike for the liquid form of platelet-rich plasma, platelet counts cannot be directly determined in solid fibrin clots and are instead calculated by subtracting the counts in other liquid or semi-clotted fractions from those in whole blood samples. Having long suspected the validity of this method, we herein examined the possible loss of platelets in the preparation process. Blood samples collected from healthy male donors were immediately centrifuged for advanced platelet-rich fibrin (A-PRF) and concentrated growth factors (CGF) according to recommended centrifugal protocols. Blood cells in liquid and semi-clotted fractions were directly counted. Platelets aggregated on clot surfaces were observed by scanning electron microscopy. A higher centrifugal force increased the numbers of platelets and platelet aggregates in the liquid red blood cell fraction and the semi-clotted red thrombus in the presence and absence of the anticoagulant, respectively. Nevertheless, the calculated platelet counts in A-PRF/CGF preparations were much higher than expected, rendering the currently accepted subtraction method inaccurate for determining platelet counts in fibrin clots. To ensure the quality of solid types of platelet concentrates chairside in a timely manner, a simple and accurate platelet-counting method should be developed immediately. View Full-Text
Keywords: fractionation; platelets; platelet-rich fibrin; concentrated growth factors; quality assurance; regenerative therapy fractionation; platelets; platelet-rich fibrin; concentrated growth factors; quality assurance; regenerative therapy
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Watanabe, T.; Isobe, K.; Suzuki, T.; Kawabata, H.; Nakamura, M.; Tsukioka, T.; Okudera, T.; Okudera, H.; Uematsu, K.; Okuda, K.; Nakata, K.; Kawase, T. An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations. Dent. J. 2017, 5, 7.

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