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Dent. J. 2015, 3(4), 111-122; doi:10.3390/dj3040111

Mineralization of Early Stage Carious Lesions In Vitro—A Quantitative Approach

1
Biomaterials Science Center, University of Basel, Gewerbestrasse 14, 4123 Allschwil, Switzerland
2
University of Applied Sciences and Arts Northwestern Switzerland FHNW, Gründenstrasse 40, 4132 Muttenz, Switzerland
*
Author to whom correspondence should be addressed.
Academic Editor: Tolga F. Tozum
Received: 31 July 2015 / Accepted: 5 October 2015 / Published: 10 October 2015
(This article belongs to the Special Issue Tomography in Dentistry)
View Full-Text   |   Download PDF [784 KB, uploaded 10 October 2015]   |  

Abstract

Micro computed tomography has been combined with dedicated data analysis for the in vitro quantification of sub-surface enamel lesion mineralization. Two artificial white spot lesions, generated on a human molar crown in vitro, were examined. One lesion was treated with a self-assembling peptide intended to trigger nucleation of hydroxyapatite crystals. We non-destructively determined the local X-ray attenuation within the specimens before and after treatment. The three-dimensional data was rigidly registered. Three interpolation methods, i.e., nearest neighbor, tri-linear, and tri-cubic interpolation were evaluated. The mineralization of the affected regions was quantified via joint histogram analysis, i.e., a voxel-by-voxel comparison of the tomography data before and after mineralization. After ten days incubation, the mean mineralization coefficient reached 35.5% for the peptide-treated specimen compared to 11.5% for the control. This pilot study does not give any evidence for the efficacy of peptide treatment nor allows estimating the necessary number of specimens to achieve significance, but shows a sound methodological approach on the basis of the joint histogram analysis. View Full-Text
Keywords: enamel caries; mineralization; demineralization; self-assembling peptide; image registration; micro computed tomography; joint histogram enamel caries; mineralization; demineralization; self-assembling peptide; image registration; micro computed tomography; joint histogram
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Deyhle, H.; Dziadowiec, I.; Kind, L.; Thalmann, P.; Schulz, G.; Müller, B. Mineralization of Early Stage Carious Lesions In Vitro—A Quantitative Approach. Dent. J. 2015, 3, 111-122.

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Dent. J. EISSN 2304-6767 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
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