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Photonics 2016, 3(4), 56; doi:10.3390/photonics3040056

Characterization of SPAD Array for Multifocal High-Content Screening Applications

1
Department of Engineering Physics, McMaster University, Hamilton, ON L8S 4L7, Canada
2
Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano; 20133 Milano, Italy
3
School of Biomedical Engineering, McMaster University, Hamilton, ON L8S 4L7, Canada
4
Department of Electrical and Computer Engineering, McMaster University; Hamilton, ON L8S 4L7, Canada
*
Author to whom correspondence should be addressed.
Received: 26 September 2016 / Revised: 24 October 2016 / Accepted: 26 October 2016 / Published: 31 October 2016
(This article belongs to the Special Issue Advanced Photodetectors Devices and Technologies)
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Abstract

Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime. Here, a 32 × 1 single-photon avalanche diode (SPAD) array was explored as a detector for fluorescence lifetime imaging (FLIM) in HCS. Device parameters and characterization results were interpreted in the context of the application to determine if the SPAD array could satisfy the requirements of HCS-FLIM. Fluorescence lifetime measurements were performed using a known fluorescence standard; and the recovered fluorescence lifetime matched literature reported values. The design of a theoretical 32 × 32 SPAD array was also considered as a detector for a multi-point confocal scanning microscope. View Full-Text
Keywords: single-photon avalanche diode; time-correlated single-photon counting; confocal microscopy; time-resolved imaging; high-content screening; drug discovery single-photon avalanche diode; time-correlated single-photon counting; confocal microscopy; time-resolved imaging; high-content screening; drug discovery
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Tsikouras, A.; Peronio, P.; Rech, I.; Hirmiz, N.; Deen, M.J.; Fang, Q. Characterization of SPAD Array for Multifocal High-Content Screening Applications. Photonics 2016, 3, 56.

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