Next Article in Journal
Eccentrically-Layered Active Coated Nano-Particles for Directive Near- and Far-Field Radiation
Next Article in Special Issue
Optical Nanofiber Integrated into Optical Tweezers for In Situ Fiber Probing and Optical Binding Studies
Previous Article in Journal
Fano Resonance Enhanced Nonreciprocal Absorption and Scattering of Light
Previous Article in Special Issue
Optical Fiber Tweezers Fabricated by Guided Wave Photo-Polymerization
Article Menu

Export Article

Open AccessArticle
Photonics 2015, 2(3), 758-772; doi:10.3390/photonics2030758

Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy

Departments of Physics and Biology, Biological Physical Sciences Institute (BPSI), University of York, York YO10 5DD, UK
*
Author to whom correspondence should be addressed.
Received: 15 May 2015 / Revised: 17 June 2015 / Accepted: 18 June 2015 / Published: 26 June 2015
(This article belongs to the Special Issue Optical Trapping in Biology and Nanotechnology)
View Full-Text   |   Download PDF [1631 KB, uploaded 26 June 2015]   |  

Abstract

We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and manipulation. Previously we have developed a super-resolution imaging module which, in conjunction with advanced imaging techniques such as Blinking assisted Localisation Microscopy (BaLM), achieves localisation precision of single fluorescent dye molecules bound to DNA of ~30 nm along the contour of the molecule; our work here describes developments in producing a system which combines tweezing and super-resolution fluorescence imaging. The instrument also features an acousto-optic deflector that temporally divides the laser beam to form multiple traps for high throughput statistics collection. Our motivation for developing the new tool is to enable direct observation of detailed molecular topological transformation and protein binding event localisation in a stretching/twisting mechanical assay that previously could hitherto only be deduced indirectly from the end-to-end length variation of DNA. Our approach is simple and robust enough for reproduction in the lab without the requirement of precise hardware engineering, yet is capable of unveiling the elastic and dynamic properties of filamentous molecules that have been hidden using traditional tools. View Full-Text
Keywords: optical tweezers; magnetic tweezers; super-resolution microscopy optical tweezers; magnetic tweezers; super-resolution microscopy
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhou, Z.; Miller, H.; Wollman, A.J.; Leake, M.C. Developing a New Biophysical Tool to Combine Magneto-Optical Tweezers with Super-Resolution Fluorescence Microscopy. Photonics 2015, 2, 758-772.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Photonics EISSN 2304-6732 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top