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Separations 2016, 3(4), 32; doi:10.3390/separations3040032

Dispersed Mobile-Phase Countercurrent Chromatography

Division of Life Science and Applied Genomics Center, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
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Author to whom correspondence should be addressed.
Academic Editor: Alain Berthod
Received: 27 July 2016 / Revised: 20 September 2016 / Accepted: 11 October 2016 / Published: 1 November 2016
(This article belongs to the Special Issue Countercurrent Chromatography)
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Abstract

Countercurrent distribution based on liquid–liquid partition is a powerful separation method with minimal incurrence of loss of solutes, but its industrial application has been limited by cumbersome shifting of immiscible solvents. Although centrifugation has been employed to facilitate equilibration between phases, process scaling-up remains difficult. In this study, a dispersed mobile-phase countercurrent chromatography (DMCC) method has been developed to adapt the countercurrent distribution principle to a continuous column chromatography format. Continuous solute exchange between two immiscible phases within a series of separation columns is achieved by mechanical dispersion of an influx of mobile phase into an upward stream of small droplets travelling through the columns filled with stationary phase. The diameter, length, and number of columns, and the number of stationary phases employed in the different columns can be varied to match the requisite scale and resolution of operation. Illustrations of DMCC were provided by examples of solute separations where the fractionated solutes could be collected either from the eluate of the series of columns, or from drainage of the stationary phases in the individual columns at the end of a chromatographic run. View Full-Text
Keywords: liquid–liquid partition; countercurrent chromatography; DMCC liquid–liquid partition; countercurrent chromatography; DMCC
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ho, T.Y.-C.; Xue, H. Dispersed Mobile-Phase Countercurrent Chromatography. Separations 2016, 3, 32.

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