Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Amino Acid Derivatives-Bonded Stationary Phases
2.2. Instrumentations
2.3. Liquid Chromatography
3. Results and Discussions
3.1. Evaluation of the Molecular Orientation of Organic Phases
3.2. Bonding Density of Amino Acid Derivatives on Silica Surface
3.3. Reversed Phase Liquid Chromatography
3.4. Hydrophilic Interaction Chromatography
3.5. Separation of Tocopherol Isomers
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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%C | %N | %H | C/N | Bonded Amount | ||||
---|---|---|---|---|---|---|---|---|
Found | calcd. | μmol·m−2 | wt % | TG % | ||||
Sil-Asp-2C18 | 17.05 | 0.9 | 2.86 | 18.94 | 18.86 | 0.98 | 23.32 | 23.00 |
Sil-Glu-2C18 | 13.71 | 0.7 | 2.37 | 19.59 | 19.29 | 0.77 | 18.69 | 18.20 |
Sil-Adi-2C18 | 15.36 | 0.8 | 2.66 | 19.20 | 19.71 | 0.84 | 20.88 | 22.06 |
Sil-Asp-2C1 | 14.59 | 3.5 | 2.28 | 4.17 | 4.29 | 3.68 | 33.23 | 26.19 |
Analyte | Sil-Asp-2C18 | Sil-Glu-2C18 | Sil-Adi-2C18 | ODS | |||||
---|---|---|---|---|---|---|---|---|---|
k | α | k | α | k | α | k | α | ||
cis-Stilbene | 1.01 | - | 0.99 | - | 1.03 | - | 1.74 | - | |
trans-Stilbene | 1.50 | 1.48 | 1.39 | 1.40 | 1.43 | 1.38 | 1.84 | 1.06 | |
o-Terphenyl | 1.32 | - | 0.88 | - | 1.23 | - | 2.27 | - | |
m-Terphenyl | 2.45 | 1.86 | 1.88 | 2.14 | 2.12 | 1.72 | 3.22 | 1.42 | |
p-Terphenyl | 3.14 | 2.39 | 2.16 | 2.46 | 2.50 | 2.03 | 3.26 | 1.43 | |
Triphenylene | 5.02 | 3.81 | 2.38 | 2.72 | 3.67 | 2.99 | 3.51 | 1.54 | |
Triphenylene | 5.02 | - | 2.38 | - | 3.67 | - | 3.51 | - | |
Benz[a]anthracene | 5.66 | 1.13 | 2.56 | 1.07 | 4.05 | 1.10 | 3.73 | 1.06 | |
Chrysene | 5.84 | 1.16 | 2.58 | 1.08 | 4.11 | 1.12 | 3.69 | 1.05 | |
Naphthacene | 7.90 | 1.57 | 3.11 | 1.31 | 5.08 | 1.38 | 4.26 | 1.21 |
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Noguchi, H.; Liu, T.; Takafuji, M.; Ihara, H. Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography. Separations 2016, 3, 25. https://doi.org/10.3390/separations3030025
Noguchi H, Liu T, Takafuji M, Ihara H. Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography. Separations. 2016; 3(3):25. https://doi.org/10.3390/separations3030025
Chicago/Turabian StyleNoguchi, Hiroki, Tianhang Liu, Makoto Takafuji, and Hirotaka Ihara. 2016. "Effects of Alignment of Weak Interaction Sites in Molecular Shape Recognition High-Performance Liquid Chromatography" Separations 3, no. 3: 25. https://doi.org/10.3390/separations3030025