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Processes 2015, 3(2), 384-405; doi:10.3390/pr3020384

The Application of Dielectric Spectroscopy and Biocalorimetry for the Monitoring of Biomass in Immobilized Mammalian Cell Cultures

School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland
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Academic Editor: Michael Henson
Received: 30 December 2014 / Revised: 27 April 2015 / Accepted: 29 April 2015 / Published: 7 May 2015
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Abstract

The purpose of this study was to introduce dielectric spectroscopy and biocalorimetry as monitoring methods to follow immobilised Chinese Hamster Ovary (CHO) cell culture development. The theory behind both monitoring techniques is explained and perfusion cultures are performed in a Reaction Calorimeter (eRC1 from Mettler Toledo) as an application example. The findings of this work show that dielectric spectroscopy gives highly reliable information upon the viable cell density throughout the entire culture. On the other hand, the RC1 could only provide accurate data from day 5, when the cell density exceeded 4 × 106 vcells∙mL−1 (viable cell per mL) working volume (WV). The method validation showed the limit of detection (LOD) for 1.4 L cultures to be 8.86 × 106 vcells∙mL−1, a viable cell density commonly achieved in fed-batch and the early stages of a perfusion culture. This work suggests that biocalorimetry should be possible to implement at industrial scale to monitor CHO cell cultures. View Full-Text
Keywords: mammalian cells; bioprocess monitoring; immobilized cells; dielectric spectroscopy; biocalorimetry mammalian cells; bioprocess monitoring; immobilized cells; dielectric spectroscopy; biocalorimetry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Cole, H.E.; Demont, A.; Marison, I.W. The Application of Dielectric Spectroscopy and Biocalorimetry for the Monitoring of Biomass in Immobilized Mammalian Cell Cultures. Processes 2015, 3, 384-405.

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