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Processes 2014, 2(3), 625-638; doi:10.3390/pr2030625

Light-Induced Production of An Antibody Fragment and Malaria Vaccine Antigen from Chlamydomonas reinhardtii

1
Biological and Agricultural Engineering Department, Texas A & M University, 2117 TAMU, College Station, TX 77843, USA
2
Pediatric Allergy and Immunology and the Jaffe Food Allergy Institute, Ichan School of Medicine at Mt. Sinai, New York, NY 10029, USA
*
Author to whom correspondence should be addressed.
Received: 31 May 2014 / Revised: 19 July 2014 / Accepted: 21 July 2014 / Published: 7 August 2014
(This article belongs to the Special Issue Advances in Bioseparation Engineering)
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Abstract

The eukaryotic green alga, Chlamydomonas reinhardtii, is a unique expression platform that can efficiently express complex therapeutic proteins. However, demonstrating that therapeutic molecules can be produced in quantifiable levels is essential to establish the potential of the C. reinhardtii expression system. Thus, the objective of this investigation was to determine the process conditions that could maximize C. reinhardtii biomass accumulation and induced-production of the two recombinant proteins, a single chain fragment antibody molecule (αCD22 scFv) and malaria vaccine antigen (Pfs25), produced in the chloroplast of C. reinhardtii. To achieve a higher production of recombinant proteins, cultivation variables of C. reinhardtii, such as mixing, light-induction time and intensity, nutrient depletion and culture age, were investigated and optimized. The optimal light-induction time was 24 h at a light intensity of 300 μmol m−2 s−1. Replacement of the culture media in the late exponential growth with fresh media was beneficial to the accumulation of recombinant proteins. Optimization led to increases in the accumulation of recombinant proteins by six-fold and the recombinant protein fraction in the extracted soluble protein by two-fold. View Full-Text
Keywords: single-chain antibody fragment; vaccine antigen; Pfs25; Chlamydomonas reinhardtii; cultivation; recombinant protein accumulation; light induction single-chain antibody fragment; vaccine antigen; Pfs25; Chlamydomonas reinhardtii; cultivation; recombinant protein accumulation; light induction
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Munjal, N.; Garzon-Sanabria, A.J.; Quinones, K.W.; Gregory, J.; Nikolov, Z.L. Light-Induced Production of An Antibody Fragment and Malaria Vaccine Antigen from Chlamydomonas reinhardtii. Processes 2014, 2, 625-638.

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