Sensitive Electrochemical Detection of Phosphorylated-Tau Threonine 231 in Human Serum Using Interdigitated Wave-Shaped Electrode
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Manufacturing of the IWE
2.3. Construction of the Biosensor IWE/SAM/EDC-NHS/anti–p-tau231
2.4. Investigation of the Modification of SAM on the IWE Surface
2.5. EIS Measurement
2.6. Cyclic Voltammetry (CV) Measurements
3. Results
3.1. Characterization the Modification of SAM on the IWE
3.2. Electrochemical Characterization of the Biosensor (IWE/SAM/EDC-NHS/anti–p-tau231)
3.3. Detection of p-tau231 in PBS and HS using the Biosensor
3.4. Binding Affinity, Dissociation Constant (Kd), and Selectivity and Stability of the Biosensor
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Le, H.T.N.; Cho, S. Sensitive Electrochemical Detection of Phosphorylated-Tau Threonine 231 in Human Serum Using Interdigitated Wave-Shaped Electrode. Biomedicines 2022, 10, 10. https://doi.org/10.3390/biomedicines10010010
Le HTN, Cho S. Sensitive Electrochemical Detection of Phosphorylated-Tau Threonine 231 in Human Serum Using Interdigitated Wave-Shaped Electrode. Biomedicines. 2022; 10(1):10. https://doi.org/10.3390/biomedicines10010010
Chicago/Turabian StyleLe, Hien T. Ngoc, and Sungbo Cho. 2022. "Sensitive Electrochemical Detection of Phosphorylated-Tau Threonine 231 in Human Serum Using Interdigitated Wave-Shaped Electrode" Biomedicines 10, no. 1: 10. https://doi.org/10.3390/biomedicines10010010