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Chemosensors 2018, 6(3), 26; https://doi.org/10.3390/chemosensors6030026

Lifetime and Fluorescence Quantum Yield of Two Fluorescein-Amino Acid-Based Compounds in Different Organic Solvents and Gold Colloidal Suspensions

1
BIOSCOPE Group, LAQV-REQUIMTE, Chemistry Department, Faculty of Science and Technology, University NOVA of Lisbon, 2829-516 Caparica, Portugal
2
Instituto de Física, Universidade Federal de Uberlândia-UFU, Av. João Naves de Ávila 2121, 38400-902 Uberlândia, Brazil
3
Instituto de Química, Universidade de São Paulo-USP, Av. Prof. Lineu Prestes 748, CxP 26077, 05508-000 São Paulo, Brazil
4
Proteomass Scientific Society, Rua dos Inventores, Madan Park, 2829-516 Caparica, Portugal
*
Author to whom correspondence should be addressed.
Received: 11 May 2018 / Revised: 28 June 2018 / Accepted: 28 June 2018 / Published: 30 June 2018
(This article belongs to the Special Issue New insides in Fluorescent and Colorimetric Probes)
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Abstract

Fluorescein and its derivatives are yellowish-green emitting dyes with outstanding potential in advanced molecular probes for different applications. In this work, two fluorescent compounds containing fluorescein and an amino acid residue (compounds 1 and 2) were photophysically explored. Compounds 1 and 2 were previously synthesized via ester linkage between fluorescein ethyl ester and Boc-ser (TMS)-OH or Boc-cys(4-methyl benzyl)-OH. Studies on the time-resolved fluorescence lifetime and relative fluorescence quantum yield (φ) were performed for 1 and 2 diluted in acetonitrile, ethanol, dimethyl sulfoxide, and tetrahydrofuran solvents. The discussion considered the dielectric constants of the studied solvents (range between 7.5 and 47.2) and the photophysical parameters. The results of the lifetime and φ were compared with those obtained for compounds 1, 2 and fluorescein without an amino acid residue in alkaline aqueous solutions. Moreover, as a preliminary result compound 2 (S-cysteine) was tested in the presence of gold nanoparticles as an aggregation colorimetric probe. View Full-Text
Keywords: fluorescein; lifetime; fluorescence quantum yield; gold nanoparticles fluorescein; lifetime; fluorescence quantum yield; gold nanoparticles
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Pilla, V.; Gonçalves, A.C.; Dos Santos, A.A.; Lodeiro, C. Lifetime and Fluorescence Quantum Yield of Two Fluorescein-Amino Acid-Based Compounds in Different Organic Solvents and Gold Colloidal Suspensions. Chemosensors 2018, 6, 26.

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