Healthcare 2013, 1(1), 64-83; doi:10.3390/healthcare1010064

Detection and Discrimination of Non-Melanoma Skin Cancer by Multimodal Imaging

1email, 1email, 1email, 1email, 2email, 2, 2email, 1,3email and 1,3,* email
Received: 6 August 2013; in revised form: 30 September 2013 / Accepted: 30 September 2013 / Published: 17 October 2013
(This article belongs to the Special Issue Melanoma and Neoplasms of Skin)
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Abstract: Non-melanoma skin cancer (NMSC) belongs to the most frequent human neoplasms. Its exposed location facilitates a fast ambulant treatment. However, in the clinical practice far more lesions are removed than necessary, due to the lack of an efficient pre-operational examination procedure: Standard imaging methods often do not provide a sufficient spatial resolution. The demand for an efficient in vivo imaging technique might be met in the near future by non-linear microscopy. As a first step towards this goal, the appearance of NMSC in various microspectroscopic modalities has to be defined and approaches have to be derived to distinguish healthy skin from NMSC using non-linear optical microscopy. Therefore, in this contribution the appearance of ex vivo NMSC in a combination of coherent anti-Stokes Raman scattering (CARS), second harmonic generation (SHG) and two photon excited fluorescence (TPEF) imaging—referred as multimodal imaging—is described. Analogous to H&E staining, an overview of the distinct appearances and features of basal cell and squamous cell carcinoma in the complementary modalities is derived, and is expected to boost in vivo studies of this promising technological approach.
Keywords: CARS; TPEF; SHG; multimodal imaging; coherent Raman scattering; skin cancer; basal cell carcinoma; squamous cell carcinoma; non-melanoma skin cancer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Heuke, S.; Vogler, N.; Meyer, T.; Akimov, D.; Kluschke, F.; Röwert-Huber, H.-J.; Lademann, J.; Dietzek, B.; Popp, J. Detection and Discrimination of Non-Melanoma Skin Cancer by Multimodal Imaging. Healthcare 2013, 1, 64-83.

AMA Style

Heuke S, Vogler N, Meyer T, Akimov D, Kluschke F, Röwert-Huber H-J, Lademann J, Dietzek B, Popp J. Detection and Discrimination of Non-Melanoma Skin Cancer by Multimodal Imaging. Healthcare. 2013; 1(1):64-83.

Chicago/Turabian Style

Heuke, Sandro; Vogler, Nadine; Meyer, Tobias; Akimov, Denis; Kluschke, Franziska; Röwert-Huber, Hans-Joachim; Lademann, Jürgen; Dietzek, Benjamin; Popp, Jürgen. 2013. "Detection and Discrimination of Non-Melanoma Skin Cancer by Multimodal Imaging." Healthcare 1, no. 1: 64-83.

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