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Proteomes 2017, 5(4), 32; doi:10.3390/proteomes5040032

A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef

1
Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa
2
Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland
*
Author to whom correspondence should be addressed.
Received: 22 June 2017 / Revised: 20 October 2017 / Accepted: 10 November 2017 / Published: 15 November 2017
(This article belongs to the Special Issue Plant Proteomics 2017)
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Abstract

The orphan crop, Eragrostis tef, was subjected to controlled drought conditions to observe the physiological parameters and proteins changing in response to dehydration stress. Physiological measurements involving electrolyte leakage, chlorophyll fluorescence and ultra-structural analysis showed tef plants tolerated water loss to 50% relative water content (RWC) before adverse effects in leaf tissues were observed. Proteomic analysis using isobaric tag for relative and absolute quantification (iTRAQ) mass spectrometry and appropriate database searching enabled the detection of 5727 proteins, of which 211 proteins, including a number of spliced variants, were found to be differentially regulated with the imposed stress conditions. Validation of the iTRAQ dataset was done with selected stress-related proteins, fructose-bisphosphate aldolase (FBA) and the protective antioxidant proteins, monodehydroascorbate reductase (MDHAR) and peroxidase (POX). Western blot analyses confirmed protein presence and showed increased protein abundance levels during water deficit while enzymatic activity for FBA, MDHAR and POX increased at selected RWC points. Gene ontology (GO)-term enrichment and analysis revealed terms involved in biotic and abiotic stress response, signaling, transport, cellular homeostasis and pentose metabolic processes, to be enriched in tef upregulated proteins, while terms linked to reactive oxygen species (ROS)-producing processes under water-deficit, such as photosynthesis and associated light harvesting reactions, manganese transport and homeostasis, the synthesis of sugars and cell wall catabolism and modification, to be enriched in tef downregulated proteins. View Full-Text
Keywords: tef; drought-stress; functional enrichment analysis; GO-term; iTRAQ; physiological characterisation; quantitative proteomics; stress-responsive proteins tef; drought-stress; functional enrichment analysis; GO-term; iTRAQ; physiological characterisation; quantitative proteomics; stress-responsive proteins
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kamies, R.; Farrant, J.M.; Tadele, Z.; Cannarozzi, G.; Rafudeen, M.S. A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes 2017, 5, 32.

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