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Proteomes 2017, 5(3), 17; doi:10.3390/proteomes5030017

Tissue Specific Labeling in Proteomics

1
Mass-Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine, 13125 Berlin, Germany
2
Berlin School of Integrative Oncology (BSIO), Charité-Universitätsmedizin Berlin, 13353 Berlin, Germany
3
Proteome and Genome Research Laboratory, Luxembourg Institute of Health, 1272 Strassen, Luxembourg
*
Author to whom correspondence should be addressed.
Received: 15 June 2017 / Revised: 13 July 2017 / Accepted: 14 July 2017 / Published: 18 July 2017
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Abstract

Mass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in multicellular organisms and tissues poses a significant challenge. Isolating a subset of cells from tissues requires mechanical and biochemical separation or sorting, a process which can alter cellular signaling, and thus, the composition of the proteome. Recently, several approaches for cell selective labeling of proteins, that include bioorthogonal amino acids, biotinylating enzymes, and genetic tools, have been developed. These tools facilitate the selective labeling of proteins, their interactome, or of specific cell types within a tissue or an organism, while avoiding the difficult and contamination-prone biochemical separation of cells from the tissue. In this review, we give an overview of existing techniques and their application in cell culture models and whole animals. View Full-Text
Keywords: quantitative proteomics; stable isotope labeling; tissue; amino acid analog; biotinylation; bioorthogonal; multicellular; SILAC; APEX; BioID quantitative proteomics; stable isotope labeling; tissue; amino acid analog; biotinylation; bioorthogonal; multicellular; SILAC; APEX; BioID
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ramberger, E.; Dittmar, G. Tissue Specific Labeling in Proteomics. Proteomes 2017, 5, 17.

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