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Proteomes 2017, 5(1), 6; doi:10.3390/proteomes5010006

De Novo Sequencing of Top-Down Tandem Mass Spectra: A Next Step towards Retrieving a Complete Protein Sequence

1
Center for Algorithmic Biotechnology, Institute of Translational Biomedicine, Saint Petersburg State University, 7-9 Universitetskaya nab., St. Petersburg 199034, Russia
2
Department of Mathematical and Information Technologies, Saint Petersburg Academic University, 8/3 Khlopina st., St. Petersburg 194021, Russia
Academic Editors: Jacek R. Wisniewski, Jens Coorssen and Alfred L. Yergey
Received: 10 November 2016 / Revised: 30 January 2017 / Accepted: 4 February 2017 / Published: 8 February 2017
View Full-Text   |   Download PDF [278 KB, uploaded 8 February 2017]   |  

Abstract

De novo sequencing of tandem (MS/MS) mass spectra represents the only way to determine the sequence of proteins from organisms with unknown genomes, or the ones not directly inscribed in a genome—such as antibodies, or novel splice variants. Top-down mass spectrometry provides new opportunities for analyzing such proteins; however, retrieving a complete protein sequence from top-down MS/MS spectra still remains a distant goal. In this paper, we review the state-of-the-art on this subject, and enhance our previously developed Twister algorithm for de novo sequencing of peptides from top-down MS/MS spectra to derive longer sequence fragments of a target protein. View Full-Text
Keywords: top-down mass spectrometry; de novo sequencing; tag convolution top-down mass spectrometry; de novo sequencing; tag convolution
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Vyatkina, K. De Novo Sequencing of Top-Down Tandem Mass Spectra: A Next Step towards Retrieving a Complete Protein Sequence. Proteomes 2017, 5, 6.

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