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Proteomes 2016, 4(4), 33; doi:10.3390/proteomes4040033

Towards the Full Realization of 2DE Power

1
Institute of Biomedical Chemistry, Pogodinskaya 10, Moscow 119121, Russia
2
B. P. Konstantinov Petersburg Nuclear Physics Institute, National Research Center “Kurchatov Institute”, Leningrad region, Gatchina 188300, Russia
Academic Editors: Jens Coorssen, Alfred L. Yergey and Jacek R. Wisniewski
Received: 25 September 2016 / Revised: 3 November 2016 / Accepted: 9 November 2016 / Published: 15 November 2016
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Abstract

Here, approaches that allow disclosure of the information hidden inside and outside of two-dimensional gel electrophoresis (2DE) are described. Experimental identification methods, such as mass spectrometry of high resolution and sensitivity (MALDI-TOF MS and ESI LC-MS/MS) and immunodetection (Western and Far-Western) in combination with bioinformatics (collection of all information about proteoforms), move 2DE to the next level of power. The integration of these technologies will promote 2DE as a powerful methodology of proteomics technology. View Full-Text
Keywords: two-dimensional gel electrophoresis; proteoforms; mass-spectrometry; bioinformatics two-dimensional gel electrophoresis; proteoforms; mass-spectrometry; bioinformatics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Naryzhny, S. Towards the Full Realization of 2DE Power. Proteomes 2016, 4, 33.

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