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Plants 2018, 7(2), 27; https://doi.org/10.3390/plants7020027

Identification of Leaf Promoters for Use in Transgenic Wheat

1
School of Biological Sciences, Wivenhoe Park, University of Essex, Colchester CO4 3SQ, UK
2
Biotechnology Department, Biological Sciences College, Taif University, At Taif 26571, Saudi Arabia
3
Rothamsted Research, West Common, Harpenden, Hertfordshire AL5 2JQ, UK
4
Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK
5
Genetics, Genomics and Breeding, NIAB EMR, New Road, East Malling ME19 6BJ, UK
*
Authors to whom correspondence should be addressed.
Received: 2 December 2017 / Revised: 6 March 2018 / Accepted: 23 March 2018 / Published: 28 March 2018
(This article belongs to the Special Issue Crop Enhancement for Global Food Security)
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Abstract

Wheat yields have plateaued in recent years and given the growing global population there is a pressing need to develop higher yielding varieties to meet future demand. Genetic manipulation of photosynthesis in elite wheat varieties offers the opportunity to significantly increase yields. However, the absence of a well-defined molecular tool-box of promoters to manipulate leaf processes in wheat hinders advancements in this area. Two promoters, one driving the expression of sedoheptulose-1,7-bisphosphatase (SBPase) and the other fructose-1,6-bisphosphate aldolase (FBPA) from Brachypodium distachyon were identified and cloned into a vector in front of the GUS reporter gene. Both promoters were shown to be functionally active in wheat in both transient assays and in stably transformed wheat plants. Analysis of the stable transformants of wheat (cv. Cadenza) showed that both promoters controlled gus expression throughout leaf development as well as in other green tissues. The availability of these promoters provides new tools for the expression of genes in transgenic wheat leaves and also paves the way for multigene manipulation of photosynthesis to improve yields. View Full-Text
Keywords: promoter; wheat; tissue specific; photosynthesis; reporter gene; yield promoter; wheat; tissue specific; photosynthesis; reporter gene; yield
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Alotaibi, S.S.; Sparks, C.A.; Parry, M.A.J.; Simkin, A.J.; Raines, C.A. Identification of Leaf Promoters for Use in Transgenic Wheat. Plants 2018, 7, 27.

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