Next Article in Journal
Patterns of Growth Costs and Nitrogen Acquisition in Cytisus striatus (Hill) Rothm. and Cytisus balansae (Boiss.) Ball are Mediated by Sources of Inorganic N
Previous Article in Journal
Short-Term Response of Sasa Dwarf Bamboo to a Change of Soil Nitrogen Fertility in a Forest Ecosystem in Northern Hokkaido, Japan
Article Menu

Export Article

Open AccessArticle
Plants 2016, 5(2), 18; doi:10.3390/plants5020018

Overexpression of Arabidopsis AnnAt8 Alleviates Abiotic Stress in Transgenic Arabidopsis and Tobacco

Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India
Present Address: Central Sericultural Research and Training Institute, Central Silk Board, NH-1A, Gallandar, Srinagar 192 121, India
*
Authors to whom correspondence should be addressed.
Academic Editor: Sylvie Renault
Received: 5 November 2015 / Revised: 28 March 2016 / Accepted: 1 April 2016 / Published: 14 April 2016
View Full-Text   |   Download PDF [7324 KB, uploaded 14 April 2016]   |  

Abstract

Abiotic stress results in massive loss of crop productivity throughout the world. Because of our limited knowledge of the plant defense mechanisms, it is very difficult to exploit the plant genetic resources for manipulation of traits that could benefit multiple stress tolerance in plants. To achieve this, we need a deeper understanding of the plant gene regulatory mechanisms involved in stress responses. Understanding the roles of different members of plant gene families involved in different stress responses, would be a step in this direction. Arabidopsis, which served as a model system for the plant research, is also the most suitable system for the functional characterization of plant gene families. Annexin family in Arabidopsis also is one gene family which has not been fully explored. Eight annexin genes have been reported in the genome of Arabidopsis thaliana. Expression studies of different Arabidopsis annexins revealed their differential regulation under various abiotic stress conditions. AnnAt8 (At5g12380), a member of this family has been shown to exhibit ~433 and ~175 fold increase in transcript levels under NaCl and dehydration stress respectively. To characterize Annexin8 (AnnAt8) further, we have generated transgenic Arabidopsis and tobacco plants constitutively expressing AnnAt8, which were evaluated under different abiotic stress conditions. AnnAt8 overexpressing transgenic plants exhibited higher seed germination rates, better plant growth, and higher chlorophyll retention when compared to wild type plants under abiotic stress treatments. Under stress conditions transgenic plants showed comparatively higher levels of proline and lower levels of malondialdehyde compared to the wild-type plants. Real-Time PCR analyses revealed that the expression of several stress-regulated genes was altered in AnnAt8 over-expressing transgenic tobacco plants, and the enhanced tolerance exhibited by the transgenic plants can be correlated with altered expressions of these stress-regulated genes. Our findings suggest a role for AnnAt8 in enhancing abiotic stress tolerance at different stages of plant growth and development. View Full-Text
Keywords: annexin; seedling growth; osmotic stress; methyl viologen stress; salt stress; seed germination annexin; seedling growth; osmotic stress; methyl viologen stress; salt stress; seed germination
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Yadav, D.; Ahmed, I.; Shukla, P.; Boyidi, P.; Kirti, P.B. Overexpression of Arabidopsis AnnAt8 Alleviates Abiotic Stress in Transgenic Arabidopsis and Tobacco. Plants 2016, 5, 18.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Plants EISSN 2223-7747 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top