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Plants 2016, 5(1), 13; doi:10.3390/plants5010013

Effect of Drought on Herbivore-Induced Plant Gene Expression: Population Comparison for Range Limit Inferences

1
Integrative Genomics Program, Black Hills State University, Spearfish, SD 57789, USA
2
Biology Department, Augustana University, Sioux Falls, SD 57197, USA
3
Biology Department, University of South Dakota, Vermillion, SD 57069, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Debora Gasperini
Received: 30 October 2015 / Revised: 4 February 2016 / Accepted: 23 February 2016 / Published: 11 March 2016
(This article belongs to the Special Issue The Jasmonate Pathway)
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Abstract

Low elevation “trailing edge” range margin populations typically face increases in both abiotic and biotic stressors that may contribute to range limit development. We hypothesize that selection may act on ABA and JA signaling pathways for more stable expression needed for range expansion, but that antagonistic crosstalk prevents their simultaneous co-option. To test this hypothesis, we compared high and low elevation populations of Boechera stricta that have diverged with respect to constitutive levels of glucosinolate defenses and root:shoot ratios; neither population has high levels of both traits. If constraints imposed by antagonistic signaling underlie this divergence, one would predict that high constitutive levels of traits would coincide with lower plasticity. To test this prediction, we compared the genetically diverged populations in a double challenge drought-herbivory growth chamber experiment. Although a glucosinolate defense response to the generalist insect herbivore Spodoptera exigua was attenuated under drought conditions, the plastic defense response did not differ significantly between populations. Similarly, although several potential drought tolerance traits were measured, only stomatal aperture behavior, as measured by carbon isotope ratios, was less plastic as predicted in the high elevation population. However, RNAseq results on a small subset of plants indicated differential expression of relevant genes between populations as predicted. We suggest that the ambiguity in our results stems from a weaker link between the pathways and the functional traits compared to transcripts. View Full-Text
Keywords: drought; herbivory; glucosinolates; carbon isotope ratio; gene expression drought; herbivory; glucosinolates; carbon isotope ratio; gene expression
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gill, G.S.; Haugen, R.; Matzner, S.L.; Barakat, A.; Siemens, D.H. Effect of Drought on Herbivore-Induced Plant Gene Expression: Population Comparison for Range Limit Inferences. Plants 2016, 5, 13.

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