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Plants 2015, 4(2), 267-283; doi:10.3390/plants4020267

Certain Malvaceae Plants Have a Unique Accumulation of myo-Inositol 1,2,4,5,6-Pentakisphosphate

1
Plant and Microbial Biology, Campus Box 7612, North Carolina State University, Raleigh, NC 27695, USA
2
Biochemistry, Virginia Polytechnic and State University, Blacksburg, VA 24061, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Francesca Sparvoli, Roberto Pilu and Eleonora Cominelli
Received: 10 April 2015 / Accepted: 19 May 2015 / Published: 29 May 2015
(This article belongs to the Special Issue Phytic Acid Pathway and Breeding in Plants)
View Full-Text   |   Download PDF [885 KB, uploaded 29 May 2015]   |  

Abstract

Methods used to quantify inositol phosphates in seeds lack the sensitivity and specificity necessary to accurately detect the lower concentrations of these compounds contained in the leaves of many plants. In order to measure inositol hexakisphosphate (InsP6) and inositol pentakisphosphate (InsP5) levels in leaves of different plants, a method was developed to concentrate and pre-purify these compounds prior to analysis. Inositol phosphates were extracted from leaves with diluted HCl and concentrated on small anion exchange columns. Reversed-phase solid phase extraction cartridges were used to remove compounds that give peaks that sometimes interfere during HPLC. The method permitted the determination of InsP6 and InsP5 concentrations in leaves as low as 10 µM and 2 µM, respectively. Most plants analyzed contained a high ratio of InsP6 to InsP5. In contrast, certain members of the Malvaceae family, such as cotton (Gossypium) and some hibiscus (Hibiscus) species, had a preponderance of InsP5. Radiolabeling of cotton seedlings also showed increased amounts of InsP5 relative to InsP6. Why some Malvaceae species exhibit a reversal of the typical ratios of these inositol phosphates is an intriguing question for future research. View Full-Text
Keywords: cotton; inositol phosphates; phytic acid; pentakisphosphate; hexakisphosphate; Malvaceae; phosphate; phytate cotton; inositol phosphates; phytic acid; pentakisphosphate; hexakisphosphate; Malvaceae; phosphate; phytate
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Phillippy, B.Q.; Perera, I.Y.; Donahue, J.L.; Gillaspy, G.E. Certain Malvaceae Plants Have a Unique Accumulation of myo-Inositol 1,2,4,5,6-Pentakisphosphate. Plants 2015, 4, 267-283.

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