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Plants 2015, 4(2), 183-195; doi:10.3390/plants4020183

Determining the Composition of Lignins in Different Tissues of Silver Birch

Department of Biosciences, Division of Plant Biology, P.O. Box 65, University of Helsinki, FI-00014 Helsinki, Finland
Natural Resources Institute Finland, Jokiniemenkuja 1, Fi-01300 Vantaa, Finland
Institute of Biotechnology, P.O. Box 65, Helsinki University, FI-00014 Helsinki, Finland
Author to whom correspondence should be addressed.
Academic Editor: Philip Harris
Received: 5 December 2014 / Revised: 9 February 2015 / Accepted: 23 March 2015 / Published: 9 April 2015
(This article belongs to the Special Issue Plant Cell Walls: Chemical and Metabolic Analysis)
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Quantitative and qualitative lignin analyses were carried out on material from the trunks of silver birch (Betula pendula Roth) trees. Two types of material were analyzed. First, whole birch trunk pieces were cryosectioned into cork cambium, non-conductive phloem, the cambial zone (conductive phloem, cambium and differentiating xylem), lignified xylem and the previous year’s xylem; material that would show differences in lignin amount and quality. Second, clonal material from one natural birch population was analyzed to show variations between individuals and between the lignin analysis methods. The different tissues showed marked differences in lignin amount and the syringyl:guaiacyl (S/G) ratio. In the non-conductive phloem tissue containing sclereids, the S/G ratio was very low, and typical for phloem fibers and in the newly-formed xylem, as well as in the previous year’s xylem, the ratio lay between five and seven, typical for broadleaf tree xylem. Clonal material consisting of 88 stems was used to calculate the S/G ratios from the thioacidolysis and CuO methods, which correlated positively with an R2 value of 0.43. Comparisons of the methods indicate clearly that the CuO method is a good alternative to study the monomeric composition and S/G ratio of wood lignins. View Full-Text
Keywords: acetyl bromide; Betula pendula; cupric oxide; lignin analysis methods; phloem; thioacidolysis; xylem acetyl bromide; Betula pendula; cupric oxide; lignin analysis methods; phloem; thioacidolysis; xylem

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Fagerstedt, K.V.; Saranpää, P.; Tapanila, T.; Immanen, J.; Serra, J.A.A.; Nieminen, K. Determining the Composition of Lignins in Different Tissues of Silver Birch. Plants 2015, 4, 183-195.

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