Quantification of (1→4)-β-d-Galactans in Compression Wood Using an Immuno-Dot Assay
Abstract
:1. Introduction
2. Results
2.1. Assays Using Reference Lupin Seed (1→4)-β-Galactans
2.2. Assays Using Extracts of Radiata Pine Wood
Tree | Amounts of (1→4)-β-Galactans Extracted Without Delignification | Amounts of (1→4)-β-Galactans Extracted after Delignification | ||||
---|---|---|---|---|---|---|
OW | CW | CW/OW | OW | CW | CW/OW | |
Mean ± SE | Mean ± SE | Mean ± SE | Mean ± SE | |||
Tree 1 | 0.03 ± 0.00 | 0.35 ± 0.03 | 11.7 | 0.38 ± 0.09 | 9.95 ± 1.39 | 26.2 |
Tree 2 | 0.02 ± 0.00 | 0.01 ± 0.00 | 0.5 | 0.60 ± 0.11 | 8.34 ± 0.60 | 14.0 |
Tree 3 | 0.01 ± 0.00 | 0.01 ± 0.00 | 1.0 | 0.42 ± 0.08 | 8.25 ± 0.02 | 19.5 |
Tree 4 | 0.03 ± 0.00 | 0.02 ± 0.00 | 0.7 | 0.60 ± 0.03 | 6.63 ± 0.95 | 11.1 |
Tree 5 | 0.01 ± 0.00 | 0.02 ± 0.00 | 2.0 | 0.39 ± 0.02 | 5.92 ± 0.76 | 15.3 |
Tree 6 | 0.03 ± 0.00 | 0.04 ± 0.00 | 1.3 | 0.22 ± 0.04 | 4.27 ± 0.50 | 19.5 |
Tree 7 | 0.03 ± 0.00 | 0.03 ± 0.00 | 1.0 | 0.74 ± 0.08 | 3.14 ± 0.10 | 4.2 |
2.3. Monosaccharide Compositions of Radiata Pine Wood
Monosaccharides | |||||||
---|---|---|---|---|---|---|---|
Arabinose | Xylose | Galactose | Glucose | Mannose | CW/OW Galactose | ||
Tree | Wood Type | Mean ± SE | Mean ± SE | Mean ± SE | Mean ± SE | Mean ± SE | |
Tree 1 | OW | 10.2 ± 0.53 | 32.2 ± 0.12 | 8.4 ± 0.07 | 13.6 ± 0.26 | 35.6 ± 0.24 | 6.3 |
CW | 4.7 ± 0.36 | 15.3 ± 0.29 | 52.7 ± 0.25 | 11.4 ± 0.11 | 16.0 ± 0.28 | ||
Tree 2 | OW | 13.2 ± 0.23 | 35.8 ± 0.47 | 10.5 ± 0.20 | 9.4 ± 0.30 | 31.2 ± 0.18 | 5.5 |
CW | 5.8 ± 0.06 | 13.1 ± 0.13 | 56.9 ± 0.49 | 9.8 ± 0.23 | 14.3 ± 0.25 | ||
Tree 3 | OW | 12.0 ± 0.33 | 38.7 ± 0.11 | 7.7 ± 0.10 | 10.1 ± 0.37 | 31.8 ± 0.46 | 7.6 |
CW | 5.3 ± 0.26 | 11.4 ± 0.17 | 58.5 ± 0.28 | 12.2 ± 0.27 | 12.6 ± 0.29 | ||
Tree 4 | OW | 13.3 ± 0.35 | 31.4 ± 0.71 | 10.2 ± 0.17 | 12.4 ± 0.25 | 32.7 ± 1.03 | 4.9 |
CW | 6.5 ± 0.24 | 15.9 ± 0.18 | 49.3 ± 0.12 | 10.4 ± 0.34 | 17.8 ± 0.78 | ||
Tree 5 | OW | 10.2 ± 1.44 | 30.4 ± 0.61 | 10.5 ± 0.05 | 13.1 ± 0.31 | 35.8 ± 0.89 | 4.6 |
CW | 7.6 ± 0.29 | 15.7 ± 0.08 | 47.7 ± 0.89 | 11.9 ± 0.16 | 17.3 ± 0.39 | ||
Tree 6 | OW | 12.3 ± 0.21 | 29.5 ± 0.31 | 7.9 ± 0.08 | 12.5 ± 0.23 | 37.8 ± 0.19 | 6.8 |
CW | 6.8 ± 0.02 | 11.9 ± 0.07 | 53.7 ± 0.41 | 11.2 ± 0.29 | 16.5 ± 0.11 | ||
Tree 7 | OW | 10.2 ± 0.90 | 34.4 ± 0.35 | 8.9 ± 0.09 | 11.1 ± 0.23 | 35.4 ± 0.29 | 4.3 |
CW | 7.4 ± 0.13 | 18.9 ± 0.39 | 38.1 ± 1.46 | 11.3 ± 0.29 | 24.4 ± 0.85 |
3. Discussion
4. Experimental
4.1. Wood Samples
4.2. Extraction of Non-Cellulosic Polysaccharides from the Wood Samples
4.3. Immuno-Dot Quantification of (1→4)-β-Galactans
4.4. Neutral Monosaccharide Compositions of the Wood Samples
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Chavan, R.R.; Fahey, L.M.; Harris, P.J. Quantification of (1→4)-β-d-Galactans in Compression Wood Using an Immuno-Dot Assay. Plants 2015, 4, 29-43. https://doi.org/10.3390/plants4010029
Chavan RR, Fahey LM, Harris PJ. Quantification of (1→4)-β-d-Galactans in Compression Wood Using an Immuno-Dot Assay. Plants. 2015; 4(1):29-43. https://doi.org/10.3390/plants4010029
Chicago/Turabian StyleChavan, Ramesh R., Leona M. Fahey, and Philip J. Harris. 2015. "Quantification of (1→4)-β-d-Galactans in Compression Wood Using an Immuno-Dot Assay" Plants 4, no. 1: 29-43. https://doi.org/10.3390/plants4010029