Genome-Wide Analysis of C-Repeat Binding Factor Gene Family in Capsicum baccatum and Functional Exploration in Low-Temperature Response
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization Analysis of the CBF Genes in C. baccatum
2.2. Evolutionary Analysis of CbCBF Proteins
2.3. Conserved Motif Analysis of CbCBF Genes
2.4. Cis-Acting Element Analysis of CbCBF Genes
2.5. Collinearity Analysis and Chromosomal Localization of CbCBF Genes
2.6. Tissue-Specific Expression Patterns of CbCBF Genes
2.7. Expression Patterns of CbCBF Genes in Cold Stress
2.8. Virus-Induced Gene Silencing in C. baccatum
2.9. Silencing the CbCBF3 Gene Reduces Cold Resistance in C. baccatum
2.10. Protein Interaction Network of CbCBF3 in C. baccatum
3. Discussion
4. Materials and Methods
4.1. Identification of CBF Genes in C. baccatum
4.2. Evolutionary Analysis of CBF Proteins
4.3. Genes Conserved Motifs Analysis
4.4. Cis-Acting Element Prediction
4.5. Collinearity Analysis
4.6. Plant Materials and Treatments
4.7. Functional Analysis of CbCBF3 Based on VIGS
4.8. Virus Vector Construction and qRT-PCR
4.9. Physiological Parameter Determination
4.10. Interaction Network Analysis of CbCBF3
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gene Name | Gene ID | Number of Amino Acid | Molecular Weight | Theoretical pI | Instability Index | Grand Average of Hydropathicity | Subcellular Localization |
---|---|---|---|---|---|---|---|
CbCBF1 | Cbp01g28680 | 162 | 18,402.47 | 4.63 | 55.57 | −0.701 | Nucleus |
CbCBF2 | Cbp03g18270 | 332 | 36,252.64 | 5.96 | 62.57 | −0.534 | Cytoplasm, Nucleus |
CbCBF3 | Cbp03g18280 | 293 | 32,782.27 | 5.03 | 56.14 | −0.33 | Cytoplasm. |
CbCBF4 | Cbp09g26670 | 199 | 21,643.08 | 5.4 | 39.53 | −0.476 | Cytoplasm, Nucleus |
CbCBF5 | Cbp10g16750 | 193 | 20,203.37 | 5.68 | 50.39 | −0.462 | Nucleus |
Primer Name | Gene ID | Forward Primer Sequence (5′ –> 3′) | Reverse Primer Sequence (5′ –> 3′) |
---|---|---|---|
CbCBF1 | Cbp01g28680 | CAATTTAAGAGGAGGGCAGCTC | TGAAGAGCCGCGATTTGGAT |
CbCBF2 | Cbp03g18270 | ATGGCGGAAGGGCTAATGTT | TACCGCTTCCGGGACAAAC |
CbCBF3 | Cbp03g18280 | TCCCTACTGCTGAAATGGCG | ACTCTGATGGTCGGAAAGCC |
CbCBF4 | Cbp09g26670 | ATGCGGATCTCAACTTCCCG | ACTGCTTCTTTACGTGCGGA |
CbCBF5 | Cbp10g16750 | AGGCTGGAAATATGGCTGGG | TGCAGGAAGGTTGAGACGTG |
PDS | Cbp05g00330 | GGCTAAGGATTTCCGGCCTT | GACAAACCACCCAAACCTGC |
Actin | Cbp03g21510 | GGTCGGAATGGGACAGAAGG | GGTGCCTCCGTTAGGAGAAC |
TRV | None | TGGGAGATGATACGCTGTT | CCTAAAACTTCAGACACG |
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Yang, Y.; Cai, Q.; Luo, L.; Sun, Z.; Li, L. Genome-Wide Analysis of C-Repeat Binding Factor Gene Family in Capsicum baccatum and Functional Exploration in Low-Temperature Response. Plants 2024, 13, 549. https://doi.org/10.3390/plants13040549
Yang Y, Cai Q, Luo L, Sun Z, Li L. Genome-Wide Analysis of C-Repeat Binding Factor Gene Family in Capsicum baccatum and Functional Exploration in Low-Temperature Response. Plants. 2024; 13(4):549. https://doi.org/10.3390/plants13040549
Chicago/Turabian StyleYang, Yanbo, Qihang Cai, Li Luo, Zhenghai Sun, and Liping Li. 2024. "Genome-Wide Analysis of C-Repeat Binding Factor Gene Family in Capsicum baccatum and Functional Exploration in Low-Temperature Response" Plants 13, no. 4: 549. https://doi.org/10.3390/plants13040549