Comparative Characterization of Pseudoroegneria libanotica and Pseudoroegneria tauri Based on Their Repeatome Peculiarities
Abstract
:1. Introduction
2. Results
2.1. Repeatome Characterization
2.2. Satellite Repeats Characterization and Their Chromosomal Localization in Ps. libanotica and Ps. tauri
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Sequencing and Bioinformatics Analysis
4.3. Fluorescence In Situ Hybridization (FISH)
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Type of DNA Repeat | Ps. libanotica | Ps. tauri | Ps. spicata | |||
---|---|---|---|---|---|---|
Reads | Summarized% | Reads | Summarized% | Reads | Summarized% | |
Unclassified_repeat (conflicting evidences) | 0 | 43.38 | 0 | 39.82 | 966 | 48.76 |
|--rDNA | 0 | 0.41 | 0 | 0.22 | 0 | 0.25 |
| |--45S_rDNA | 0 | 0.37 | 0 | 0.18 | 2385 | 0.18 |
| | |--18S_rDNA | 3710 | 0.13 | 2684 | 0.11 | 0 | 0 |
| | |--25S_rDNA | 7109 | 0.24 | 1532 | 0.07 | 1538 | 0.07 |
| ′--5S_rDNA | 1267 | 0.04 | 1062 | 0.04 | 1551 | 0.07 |
|--satellite | 157,704 | 5.35 | 55,132 | 2.36 | 122,341 | 5.42 |
′--mobile element | 0 | 37.62 | 0 | 37.24 | 0 | 43.05 |
|--Class_I | 0 | 35.31 | 0 | 35.01 | 0 | 40.03 |
| |--LTR | 187,564 | 35.25 | 108,460 | 34.96 | 137,616 | 39.98 |
| | |--Ty1/Copia | 110 | 7.92 | 0 | 8.34 | 0 | 9.30 |
| | | |--Ale | 493 | 0.02 | 0 | 0 | 0 | 0 |
| | | |--Angela | 143,057 | 4.85 | 124,821 | 5.33 | 104,690 | 4.64 |
| | | |--Bianca | 166 | 0.01 | 0 | 0 | 0 | 0 |
| | | |--Ikeros | 1825 | 0.06 | 794 | 0.03 | 1172 | 0.05 |
| | | |--SIRE | 80,047 | 2.71 | 64,755 | 2.77 | 99,682 | 4.41 |
| | | |--TAR | 7413 | 0.25 | 4935 | 0.21 | 4685 | 0.2 |
| | | |--Tork | 617 | 0.02 | 0 | 0 | 0 | 0 |
| | ′--Ty3/Gypsy | 0 | 20.98 | 0 | 21.99 | 0 | 24.58 |
| | |--non-chromovirus | 0 | 14.06 | 0 | 15.89 | 0 | 17.39 |
| | | |--Athila | 305,327 | 10.35 | 288,182 | 12.31 | 263,871 | 11.69 |
| | | |--Ogre | 3420 | 0.12 | 2465 | 0.1 | 23,767 | 1.05 |
| | | ′--Retand | 105,876 | 3.59 | 81,421 | 3.48 | 104,927 | 4.65 |
| | ′--chromovirus | 0 | 6.92 | 0 | 6.1 | 0 | 7.19 |
| | |--CRM | 30,327 | 1.03 | 17,216 | 0.73 | 17,452 | 0.77 |
| | |--Tekay | 173,721 | 5.89 | 125,519 | 5.37 | 144,859 | 6.42 |
| ′--LINE | 1825 | 0.06 | 1276 | 0.05 | 1267 | 0.05 |
′--Class_II | 0 | 2.31 | 0 | 2.23 | 0 | 3.02 |
| |--EnSpm/CACTA | 59,033 | 2 | 45,535 | 1.95 | 62,034 | 2.75 |
| |--MuDR/Mutator | 5277 | 0.18 | 1668 | 0.07 | 5504 | 0.25 |
| |--PIF/Harbinger | 3464 | 0.12 | 4840 | 0.21 | 306 | 0.02 |
′--Helitron | 237 | 0.01 | 0 | 0 | 0 | 0 |
|--plastid | 38,982 | - | 40,246 | - | 16,336 | - |
′--mitochondria | 6774 | - | 0 | - | 4362 | - |
Unclassified repeat (No evidence) | 335,904 | - | 251,051 | - | 206,323 | - |
Repeat | Species of Origin | NCBI Accession | Identity to New Satellites, % | ||
---|---|---|---|---|---|
CL69 | CL101 | CL119 | |||
Sc26c38_V112 | S. cereale | KC243240.1 | xxx ** | xxx | 74.2 |
AesTR-183 | Ae. speltoides | MK283667.1 | xxx | xxx | 75.4 |
pTa-465 | T. aestivum | KC290905.1 | xxx | xxx | 77.8 |
CL131 | Ae. crassa | ON872663.1 | xxx | xxx | 79.0 |
pAcPR5 | A. cristatum | KX390696.1 | xxx | xxx | 82.5 |
BSCL156-3 | Th. bessarabicum | n/a * | xxx | xxx | 84.8 |
CL149 | Th. bessarabicum | ON872689.1 | xxx | xxx | 85.0 |
BSCL156-1 | Th. bessarabicum | n/a | xxx | xxx | 86.5 |
18-158 | Th. ponticum | n/a | xxx | xxx | 86.9 |
BSCL156-2 | Th. bessarabicum | n/a | xxx | xxx | 89.7 |
Oligo-1AL | T. aestivum | n/a | xxx | xxx | 90.0 |
CL232 | Ae. crassa | ON872668.1 | xxx | xxx | 94.7 |
CL211 | Th. bessarabicum | ON872686.1 | 71.9 | xxx | xxx |
CL239 | Ae. crassa | ON872677.1 | 82.4 | 71.4 | xxx |
oligo-6VS-57 | D. villosum | n/a | 92.5 | xxx | xxx |
oligo-7E-744 | Th. elongatum | n/a | 98.2 | 79.5 | xxx |
Spelt1 | Ae. speltoides | AY117402.1 | xxx | 68.3 | xxx |
pSp1B16.1 | Ae. speltoides | FJ594248.1 | xxx | 69.7 | xxx |
pSp1B16.3 | Ae. speltoides | FJ617549.1 | xxx | 76.3 | xxx |
pSp1B16.4 | Ae. speltoides | FJ617550.1 | xxx | 69.0 | xxx |
Tri-MS-6 | T. aestivum | EF469549.1 | xxx | 69.9 | xxx |
Repeat | Species of Origin | NCBI Accession | Identity to New Satellites, % | |||||
---|---|---|---|---|---|---|---|---|
CL82 | CL89 | CL168 | CL185 | CL192 | CL207 | |||
Oligo-1AS | A. speltoides | n/a * | xxx ** | 67.7 | xxx | xxx | xxx | 67.7 |
StLIB98 | Ps. libanotica | OL685354.1 | 76.8 | xxx | xxx | xxx | xxx | xxx |
oligo-7E-430 | Th. elongatum | n/a | 78.2 | xxx | xxx | xxx | xxx | xxx |
oligo-5D151 | T. aestivum | n/a | 81.1 | xxx | xxx | xxx | xxx | xxx |
S5 | Ps. stipifolia | n/a | 81.3 | xxx | xxx | xxx | 72.7 | xxx |
pTa-451 | T. aestivum | KC290912.1 | 87.5 | xxx | xxx | xxx | xxx | xxx |
CL149 | Th. bessarabicum | ON872689.1 | 94.4 | xxx | xxx | xxx | xxx | xxx |
pAcPR3 | A. cristatum | KX390694.1 | xxx | xxx | xxx | xxx | 82.1 | xxx |
FAT | T. aestivum | DX374230.1 | 81.2 | 74.6 | 91.7 | 82.9 | 73.0 | 90.5 |
pAs1-4, oligo-pAs1-1, pAs1 | A. speltoides | n/a | xxx | 85,7 | xxx | xxx | 83.3 | xxx |
RcAfa | Roegneria ciliaris | n/a | xxx | xxx | xxx | xxx | 82.9 | xxx |
oligo-pTa535-1 | T. aestivum | n/a | xxx | xxx | xxx | xxx | 76.2 | xxx |
CL3 | Ae. crassa | ON872662.1 | 84.4 | 85.7 | xxx | xxx | 74.4 | xxx |
CL18 | Ae. crassa | n/a | 84.2 | 75.0 | 91.7 | 91.7 | xxx | 90.5 |
ACRI_CL80 | A. cristatum | MG323513.1 | 94.4 | 70.4 | 78.0 | 74.1 | xxx | 67.1 |
CL193 | Ae. crassa | ON872676.1 | 73.5 | 76.8 | 80.4 | 82.6 | xxx | xxx |
CL148 | Th. bessarabicum | ON872688.1 | xxx | 72.1 | 76.4 | 78.5 | xxx | 68.75 |
P631 | Ae. tauschii | MK256651.1 | 85.2 | 100,0 | 82.9 | 95.5 | xxx | xxx |
P523 | Ae. tauschii | MK256655.1 | 77.2 | xxx | xxx | xxx | 100.0 | xxx |
P720 | Ae. tasuchii | MK256649.1 | 94.4 | 80.6 | xxx | xxx | 72.9 | xxx |
Repeat | Primers | Monomer Length, bp |
---|---|---|
CL69 | F: 5′-ACTACCTTTTCAAGCCACCGT-3′ R: 5′-GGAGGTCATATATGGAGACCTATTT-3′ | 178 |
CL82 | F: 5′-TGACACCATGCCAAGTTTCAT-3′ R: 5′-GTGCATGTTTAGGTCCCATGC-3′ | 503 |
CL89 | F: 5′-CACTGGGCACAACCAAAGTT-3′ R: 5′-ACAAAAGGGCTCCATGCACA-3′ | 658 |
CL101 | F: 5′-TTAAGGATGGTTTGGGCAGC-3′ R: 5′-ACCACACGTCACTCTGAAACA-3′ | 177 |
CL119 | F: 5′-CCTTTGACTTTCGCCGGAC-3′ R: 5′- CGACACGGAGGGAATCTTGC-3′ | 668 |
CL168 | F: 5′-TTTTTGTGAAGCAAGTGCCAT-3′ R: 5′-TAGAGCACACTTGCAGTTCA-3′ | 476 |
CL185 | F: 5′-CACATGGGATGCCAACTGC-3′ R: 5′-TGGTCGAAACTAGAGCACACT-3′ | 659 |
CL192 | F: 5′-TATACGCCATTGGAAGCCCC-3′ R: 5′-ACTCGTTAGCACGCCCAAAT-3′ | 339 |
CL207 | F: 5′-TTGGATGGCCACTGACCAAG-3′ R: 5′-TGGCAATTTTCAGGACCAAACT-3′ | 657 |
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Kroupin, P.Y.; Yurkina, A.I.; Ulyanov, D.S.; Karlov, G.I.; Divashuk, M.G. Comparative Characterization of Pseudoroegneria libanotica and Pseudoroegneria tauri Based on Their Repeatome Peculiarities. Plants 2023, 12, 4169. https://doi.org/10.3390/plants12244169
Kroupin PY, Yurkina AI, Ulyanov DS, Karlov GI, Divashuk MG. Comparative Characterization of Pseudoroegneria libanotica and Pseudoroegneria tauri Based on Their Repeatome Peculiarities. Plants. 2023; 12(24):4169. https://doi.org/10.3390/plants12244169
Chicago/Turabian StyleKroupin, Pavel Yu., Anna I. Yurkina, Daniil S. Ulyanov, Gennady I. Karlov, and Mikhail G. Divashuk. 2023. "Comparative Characterization of Pseudoroegneria libanotica and Pseudoroegneria tauri Based on Their Repeatome Peculiarities" Plants 12, no. 24: 4169. https://doi.org/10.3390/plants12244169