Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Husbandry
2.2. Generation of the NBT:GCaMP3 Transgenic Line
2.3. Imaging and Visual Stimulation
2.4. Image Analysis
2.5. Mapping Receptive Field Properties
2.6. Immunohistochemistry and Imaging
3. Results
3.1. Robust Expression of a Calcium Reporter in the Optic Tectum of NBT:GCaMP3 Fish
3.2. Imaging Neuronal Activity in the Optic Tectum of 3 Weeks Old Larvae
3.3. Mapping RF Properties in the Tectal Neuropil and the Cell Bodies
3.4. Imaging of Neuronal Activity in Other Brain Areas
4. Discussion
4.1. Imaging 2–3 Weeks Old Larvae
4.2. RF Properties in Late Stage Larvae
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Bergmann, K.; Meza Santoscoy, P.; Lygdas, K.; Nikolaeva, Y.; MacDonald, R.B.; Cunliffe, V.T.; Nikolaev, A. Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish. J. Dev. Biol. 2018, 6, 6. https://doi.org/10.3390/jdb6010006
Bergmann K, Meza Santoscoy P, Lygdas K, Nikolaeva Y, MacDonald RB, Cunliffe VT, Nikolaev A. Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish. Journal of Developmental Biology. 2018; 6(1):6. https://doi.org/10.3390/jdb6010006
Chicago/Turabian StyleBergmann, Katharina, Paola Meza Santoscoy, Konstantinos Lygdas, Yulia Nikolaeva, Ryan B. MacDonald, Vincent T. Cunliffe, and Anton Nikolaev. 2018. "Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish" Journal of Developmental Biology 6, no. 1: 6. https://doi.org/10.3390/jdb6010006