Next Article in Journal / Special Issue
Ethanol Exposure Causes Muscle Degeneration in Zebrafish
Previous Article in Journal
Flying the RNA Nest: Drosophila Reveals Novel Insights into the Transcriptome Dynamics of Early Development
Previous Article in Special Issue
Dynamic Tissue Rearrangements during Vertebrate Eye Morphogenesis: Insights from Fish Models
Article Menu

Export Article

Open AccessArticle
J. Dev. Biol. 2018, 6(1), 6; https://doi.org/10.3390/jdb6010006

Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish

1
Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK
2
Department of Infection, Immunity and Cardiovascular Disease, The University of Sheffield, Western Bank Sheffield, S10 2TN, UK
*
Authors to whom correspondence should be addressed.
Received: 6 February 2018 / Revised: 1 March 2018 / Accepted: 6 March 2018 / Published: 9 March 2018
(This article belongs to the Special Issue Zebrafish - A Model System for Developmental Biology Study)
View Full-Text   |   Download PDF [3381 KB, uploaded 9 March 2018]   |  

Abstract

The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7–9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages. Consequently, there is a need for an experimental paradigm to image the visual system in zebrafish larvae beyond 9 dpf. Here, we show that using NBT:GCaMP3 line allows for imaging neuronal activity in the optic tectum in late stage larvae until at least 21 dpf. Utilising this line, we have characterised the receptive field properties of tectal neurons of the 2–3 weeks old fish in the cell bodies and the neuropil. The NBT:GCaMP3 line provides a complementary approach and additional opportunities to study neuronal activity in late stage zebrafish larvae. View Full-Text
Keywords: zebrafish; calcium imaging; neuronal activity zebrafish; calcium imaging; neuronal activity
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Bergmann, K.; Meza Santoscoy, P.; Lygdas, K.; Nikolaeva, Y.; MacDonald, R.B.; Cunliffe, V.T.; Nikolaev, A. Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish. J. Dev. Biol. 2018, 6, 6.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Dev. Biol. EISSN 2221-3759 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top