Microglia Activated by Excess Cortisol Induce HMGB1 Acetylation and Neuroinflammation in the Hippocampal DG Region of Mice Following Cold Exposure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Experimental Design
2.2. Serum and Hippocampus Corticosterone Assay
2.3. Brain Tissue Collection
2.4. Immunofluorescence Analysis
2.5. Immunohistochemistry
2.6. Nissl Staining
2.7. Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) Assay
2.8. Cell Culture and Reagents
2.9. CORT Treatment
2.10. Cell Counting Kit (CCK)-8 Assay
2.11. Quantitative Real-Time (qRT-)PCR Analysis
2.12. Hippocampus Tissue and Cell Protein Extraction
2.13. Cytoplasmic and Nuclear Protein Extraction
2.14. Western Blot Analysis
2.15. Immunocytochemistry
2.16. Co-Immunoprecipitation (Co-IP)
2.17. Statistical Analysis
3. Results
3.1. CORT Levels in the Mouse Serum and Hippocampus Following Cold Exposure
3.2. Cold Exposure Increases the Number of Glial Cells in the Hippocampus
3.3. Cold Exposure Alters Hippocampus Homeostasis
3.4. Cold Exposure Increases HMGB1 and p65 Acetylation in the Hippocampus
3.5. Excess CORT Activates BV2 Cells
3.6. Excess CORT Promotes HMGB1 Activation and Secretion in BV2 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Item | Primer | Lengh (bp) |
---|---|---|
IL-1β (Forward) | 5’-GCTGCTTCCAAACCTTTGAC-3’ | 121 |
IL-1β (Reverse) | 5’-AGCTTCTCCACAGCCACAAT-3’ | |
β-actin (Forward) | 5’-GTCAGGTCATCACTATCGGCAAT-3’ | 147 |
β-actin (Reverse) | 5’-AGAGGTCTTTACGGATGTCAACGT-3’ |
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Xu, B.; Lang, L.-M.; Li, S.-Z.; Guo, J.-R.; Wang, J.-F.; Yang, H.-M.; Lian, S. Microglia Activated by Excess Cortisol Induce HMGB1 Acetylation and Neuroinflammation in the Hippocampal DG Region of Mice Following Cold Exposure. Biomolecules 2019, 9, 426. https://doi.org/10.3390/biom9090426
Xu B, Lang L-M, Li S-Z, Guo J-R, Wang J-F, Yang H-M, Lian S. Microglia Activated by Excess Cortisol Induce HMGB1 Acetylation and Neuroinflammation in the Hippocampal DG Region of Mice Following Cold Exposure. Biomolecules. 2019; 9(9):426. https://doi.org/10.3390/biom9090426
Chicago/Turabian StyleXu, Bin, Li-Min Lang, Shi-Ze Li, Jing-Ru Guo, Jian-Fa Wang, Huan-Min Yang, and Shuai Lian. 2019. "Microglia Activated by Excess Cortisol Induce HMGB1 Acetylation and Neuroinflammation in the Hippocampal DG Region of Mice Following Cold Exposure" Biomolecules 9, no. 9: 426. https://doi.org/10.3390/biom9090426