Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents, Materials, and Antibodies
2.2. Animals, Experimental Design, and Study Approval
2.3. Unilateral Ischemia-Reperfusion Injury
2.4. Plasma Biochemistry
2.5. Histology and Transmission Electron Microscopy
2.6. Immunohistochemistry and Immunofluorescence
2.7. Subcellular Fractionation
2.8. Western Blot
2.9. Enzyme-Linked Immunosorbent Assays
2.10. Statistical Analysis
3. Results
3.1. Fasting Attenuates Tubular Injury and Fibrosis Induced by Ischemia-Reperfusion Injury
3.2. Fasting Prevents Oxidative Stress Long after Ischemia-Reperfusion Injury
3.3. Fasting Protects Mitochondria Long After Ischemia-Reperfusion Injury
3.4. Fasting Protects Against Ischemia-Reperfusion Induced Endoplasmatic Reticulum Stress
3.5. Fasting Suppresses Inflammation Long After Ischemia-Reperfusion Injury
3.6. Effect of Fasting on ERK and mTOR Signaling Pathway
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Rojas-Morales, P.; Tapia, E.; León-Contreras, J.C.; González-Reyes, S.; Jiménez-Osorio, A.S.; Trujillo, J.; Pavón, N.; Granados-Pineda, J.; Hernández-Pando, R.; Sánchez-Lozada, L.G.; et al. Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury. Biomolecules 2019, 9, 404. https://doi.org/10.3390/biom9090404
Rojas-Morales P, Tapia E, León-Contreras JC, González-Reyes S, Jiménez-Osorio AS, Trujillo J, Pavón N, Granados-Pineda J, Hernández-Pando R, Sánchez-Lozada LG, et al. Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury. Biomolecules. 2019; 9(9):404. https://doi.org/10.3390/biom9090404
Chicago/Turabian StyleRojas-Morales, Pedro, Edilia Tapia, Juan Carlos León-Contreras, Susana González-Reyes, Angélica Saraí Jiménez-Osorio, Joyce Trujillo, Natalia Pavón, Jessica Granados-Pineda, Rogelio Hernández-Pando, Laura Gabriela Sánchez-Lozada, and et al. 2019. "Mechanisms of Fasting-Mediated Protection against Renal Injury and Fibrosis Development after Ischemic Acute Kidney Injury" Biomolecules 9, no. 9: 404. https://doi.org/10.3390/biom9090404