Genome-Wide Investigation of the NAC Gene Family and Its Potential Association with the Secondary Cell Wall in Moso Bamboo
Abstract
:1. Introduction
2. Results
2.1. Identification of NACs in Moso Bamboo
2.2. Gene Structure and Conserved Motif Analyses of PeNACs
2.3. Phylogenetic Analysis and Classification of PeNACs
2.4. Putative Functions of PeNACs in Moso Bamboo
2.5. Expression Patterns of PeNACs Based on Transcriptome Data of Moso Bamboo
2.6. Expression Correlation and Co-Expression Networks
2.7. Prediction of miRNA Target Sites in PeNACs
2.8. Expression Changes in Different-Height Shoots of Moso Bamboo
2.9. Transcriptional Activity Assay
3. Discussion
3.1. Conservation and Diversity of PeNACs
3.2. Homologous Genes Indicating Similar Functions
3.3. Diverse Expression Patterns of PeNACs
3.4. Complexity of SCW Biosynthesis
4. Materials and Methods
4.1. Identification and Nomenclature of PeNACs
4.2. Gene Structure, Protein Characterization, and Sequence Analyses
4.3. Phylogenetic Analysis and Functional Prediction
4.4. Tissue-Specific Expression Analysis of PeNACs Based on Transcriptome Data
4.5. Co-Expression Network of PeNACs and Subsequent GO Analysis
4.6. Plant Material Collection and cDNA Synthesis
4.7. Real-Time Quantitative PCR Analysis
4.8. Histological Analysis Method
4.9. Transcriptional Activity Analysis of PeNACs
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Shan, X.; Yang, K.; Xu, X.; Zhu, C.; Gao, Z. Genome-Wide Investigation of the NAC Gene Family and Its Potential Association with the Secondary Cell Wall in Moso Bamboo. Biomolecules 2019, 9, 609. https://doi.org/10.3390/biom9100609
Shan X, Yang K, Xu X, Zhu C, Gao Z. Genome-Wide Investigation of the NAC Gene Family and Its Potential Association with the Secondary Cell Wall in Moso Bamboo. Biomolecules. 2019; 9(10):609. https://doi.org/10.3390/biom9100609
Chicago/Turabian StyleShan, Xuemeng, Kebin Yang, Xiurong Xu, Chenglei Zhu, and Zhimin Gao. 2019. "Genome-Wide Investigation of the NAC Gene Family and Its Potential Association with the Secondary Cell Wall in Moso Bamboo" Biomolecules 9, no. 10: 609. https://doi.org/10.3390/biom9100609